The shRNA Core Facility provides reagents, expertise, and infrastructure to enable RNAi-based loss-of-function studies at scales ranging from individual genes to genome scale.  The facility houses four genome-wide arrayed shRNA libraries targeting human and mouse genes, state-of-the-art robotics to enable high-throughput lentivirus production, and high-content assay equipment for arrayed screen readouts.  Selection-based screens using pooled shRNA libraries are also supported.  In addition, human and mouse ORF libraries are available for gain-of-function studies or rescue experiments.  Expert core scientists are available to assist in planning and carrying out successful RNAi-based experiments.


  • RNAi reagents from human and mouse GIPZ and TRC libraries, supplied as:
    • Bacterial glycerol stocks
    • Plasmid DNA
    • Lentivirus
  • Screening services
    • Arrayed sets of lentiviral shRNAs
    • Genome-scale pooled library screens
    • Custom pooled library screens
  • Generation of stable cell lines expressing shRNAs or ORFs
  • Assessment of knockdown
  • Advice on RNAi experimental design and data analysis

Location and Contacts


Price Center/Block Research Pavillion 268


Scientific Director
Steven C. Almo, Ph.D.

Operations Director
John Reidhaar-Olson, Ph.D.