Pronuclear Injection Station

Pronuclear Injection

  • Individual investigators will provide the isolated DNA in a gel slice
  • We will purify the DNA
  • The facility produces 30 pups or 3 founders, whichever comes first
  • Foster mothers will be housed for 12-14 days and then the resulting pups are transferred to the investigators's room via the Institute of Animal Studies
  • Investigators must respond within 2 weeks with PCR/Southern results
  • C57 embryos are injected at an additional cost, please discuss with facility director

    Pronuclear Microinjection Form

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Lentiviral Transgenesis

  • Investigators must contact the facility and make special arrangements for lentiviral injection
  • Investigator is responsible for making the lentiviral construct, the infection and purification of the lentivirus
  • The facility will produce 30 pups or 3 founders, whichever comes first
  • Investigators must respond within 2 weeks with PCR/Southern results
  • Contact facility to specify strain

    Lentiviral Transgenesis Form

Vasectomy

  • Investigators must inform Institute of Animal Studies of procedure that will require the removal and reintroduction of animals back into their respective animal rooms (not allowed unless given permission)
  • Bring procedure candidates directly to Price Room B110
  • Facility will contact you to pick up animals after completion of procedure

    Miscellaneous Service Form

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Rederivation

  • Clear procedure and animal handling details with Institute of Animal Studies
  • Obtain any necessary training for oviduct isolation or plug identification
  • Obtain any super ovulation hormones if needed
  • Bring procedure candidates directly to Price Rm. B110
  • Facility will contact you to pick up animals after completion of procedure

    Rederivation Form

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In-vitro Fertilization

  • Clear procedure and animal handling details with Institute of Animal Studies
  • Clarify any animal needs (if necessary)
  • Obtain any super ovulation hormones if needed
  • Bring procedure candidates directly to Price Rm. B110
  • Facility will contact you to pick up animals after completion of procedure

    In-vitro Fertilization Form

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Embryo Cryopreservation

  • Obtain any necessary training for plug isolation
  • Obtain any super ovulation hormones if needed
  • Bring procedure candidates directly to Price Rm. B110
  • 20 - 30 embryos frozen per cryogenic tube
  • Facility will contact you to pick up cryogenic tubes after completion of procedure

    Embryo Cryopreservation Form

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Sperm Cryopreservation

  • Bring procedure candidates directly to Price Rm. B110
  • Two males preferred per line
  • Isolated sperm of males from same line will be mixed and divided into 12-14 cryogenic tubes
  • Facility will contact you to pick up cryogenic tubes after completion of procedure

    Sperm Cryopreservation Form

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Additional Miscellaneous Services

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Health and Safety Review of Transgenic Constructs

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