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Resources
shRNA Core Facility
Resources
Protocols and Other Information
Reagents and Information
A summary of reagent sources for lentiviral packaging, and information on protocols and controls, prepared by the shRNA Core Facility
Past RNAi User Group Presentations
An introduction to the shRNA Core Facility
John Reidhaar-Olson, Ph.D., Operations Director, shRNA Core Facility
January 25, 2012
shRNA: The Basics. A walk-through of steps and decision points for planning an shRNA experiment and ordering the appropriate reagents
Deborah Smith, Ph.D., shRNA Core Facility
February 29, 2012
Cellular and Biochemical Assays Utilizing PerkinElmer Technologies: Applications for RNAi Research
Paul Fletcher, Ph.D., Field Applications Scientist, Life Sciences & Technology, PerkinElmer Inc.
March 28, 2012
External Resources
Open Biosystems
Supplier of shRNA and ORF reagents and libraries, including the Einstein GIPZ, TRC and LentiORF libraries.
Public TRC Portal
Information about The RNAi Consortium shRNA collection.
The ORFeome Collaboration
Information about the sequence-validated human ORF clone that comprise the LentiORF library.
Sigma gene search
Portal for shRNA reagents from the TRC collection. Notes which have been validated.
GenomeRNAi
Database of phenotypes from RNAi screens in human and Drosophila cells, maintained by the German Cancer Research Center (DKFZ) of Heidelberg University.