shRNA Core Facility

Services and Pricing

For pricing details, please visit einstein.ilabsolutions.com or contact us. 

Stem Cell Institute members receive 30% off for all services.  

CRISPR services

All of our CRISPR services use lentiviral vectors, in order to increase the efficiency of the process and make it applicable to most cells types. We also use inducible Cas9 contructs to minimize undesired effects of long-term Cas9 expression, and to facilitate reversible modulation of expression when using the CRISPRa or CRISPRi systems.

For customers external to Einstein and Montefiore, prices are 50% higher than listed below, and require an MTA.  

  • Generation of polyclonal Cas9/sgRNA cell line from parental cell line$600
    • sgRNA design and cloning
    • Preparation of Cas9 and sgRNA lentivirus
    • Transduction with Cas9 lentivirus and selection on antibiotic
      • Wild-type Cas9 (for knockouts, deletions, mutations, and knockins) or dCas9-VP64 (for activation)
    • Transduction with sgRNA lentivirus and selection on blasticidin
    • Engineered cells delivered as growing cells or frozen stocks
    • Customer must induce Cas9 expression with doxycycline to effect gene editing or activation
  • Generation of polyclonal Cas9/sgRNA cell line from Cas9 cell line - $500
    • sgRNA design and cloning
    • Preparation and titering of sgRNA lentivirus
    • Transduction with sgRNA lentivirus and selection on blasticidin
    • Engineered cells delivered as growing cells or frozen stocks
    • Customer must induce Cas9 expression with doxycycline to effect gene editing or activation
  • Generation of polyclonal Cas9 cell line from parental cell line - $100
    • Transduction with Cas9 lentivirus and selection on antibiotic
    • Wild-type Cas9 (for knockouts, deletions, mutations, and knockins) or dCas9-VP64 (for activation)
  •  Production of sgRNA lentivirus - $400
    • sgRNA design and cloning
    • Preparation and titering of sgRNA lentivirus
  • Homology repair template design - $150
    • Design of template for mutation of knockin experiment
  • Cell line optimization - $150
    • Determination of transduction MOI
    • Determination of concentration of puromycin or neomycin for Cas9 vector selection
    • Determination of concentration of blasticidin for sgRNA vector selection
  • Pooled CRISPR screen - standard screen - $6,000
    • Generation of pooled lentiviral library
    • Generation of Cas9 cell line
    • Preparation of samples for next generation sequencing
    • Customer must perform the cell work and apply the selection
    • Sequencing cost is extra
    • Extra charge may be incurred for very large libraries, difficult-to-transduce cells, or large sample number
  • Pooled CRISPR screen - large screen - $9,000
    • Generation of pooled lentiviral library
    • Generation of Cas9 cell line
    • Preparation of samples for next generation sequencing
    • Customer must perform the cell work and apply the selection
    • Sequencing cost is extra
    • Applies to screens with large libraries, difficult-to-transduce cells, or large numbers of samples, where viral prep must be scaled up significantly

 

RNAi and ORF Reagents

Library_We supply shRNAs and ORFs from our libraries in these formats:

  • Lentivirus, either as viral supernatant (200 µl >106 TU/ml) ($135 per clone) or concentrated viral stock (50 µl at >108 TU/ml) ($325 per clone)  
  • Plasmid DNA ($100 per clone)  
  • Bacterial glycerol stocks ($50 per clone) 
  • Stable shRNA- or ORF-expressing cell lines ($210 per clone)  
We offer multi-clone discount on shRNAs when ordering 4 or more clones for the same gene.
Note: The shRNA Core Facility does not supply packaging plasmids or cells for generation of lentivirus. Upon request, we can provide you with information on sources for these materials. 
 
 

Screening services 

We perform gene modulation screens in two formats:

  • Custom arrayed screens. The readout can be plate-reader-based or image-based.
  • Pooled screens. The pooled format is appropriate for enrichment or dropout screens where a selection for the desired phenotype can be applied. Hits are identified by next-generation sequencing.

OperettaScreens can be performed with three different gene modulation approaches:

  • Knockdown screens (shRNA)
  • Knockout screens (CRISPR)
  • Overexpression screens (ORF)

We have equipment available for assays:  

  • Operetta High-Content Instrument: for image-based readouts in 96-well, 384-well, and other formats. Analysis software permits quantitation of a wide variety of cell parameters. $100 per hour of instrument and analysis time, 1 hour minimum. 
  • EnVision Multiplate Reader: for fluorescence, luminescence, absorbance, and other 96- or 384-well based plate reader assays. $40 per hour, 1 hour minimum. 

Expertise

We are available to consult on gene modulation experiments and offer our expertise on experimental design and data analysis.


 

Note: Restrictions may apply for external researchers (those not at Einstein, Montefiore, or Yeshiva Univesity). Please contact us for detais.

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