shRNA Core Facility

Instrumentation

Robot_2The shRNA Core Facility is equipped with state-of-the-art robotics and assay equipment, shared with the Macromolecular Therapuetics Development Facility.

Assay equipment includes:

  • EnVision Multilabel Plate Reader
    • Fluorescence intensity
    • Fluorescence polarization
    • Luminescence
    • Absorbance
    • Time-resolved fluorescence
    • AlphaLISA
     
  • Operetta High-Content Imaging System
    • Suitable for shRNA-based arrayed screens
    • Fluorescence and brightfield imaging
    • Confocal
    • Sophisticated image analysis software
    • Xenon arc lamp
    • 2x, 10x, and 20x objectives
    • Four excitation and emisison filters (blue, green, red, far red)
    • Environmental control chamber (temperature and CO2) for live-cell imaging
     

The EnVision and Operetta instruments are available for use in RNAi screening. Time on these instruments is also available for non-screening applications. Contact us for more information.

 

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