Analytical Imaging Facility

Multiphoton Olympus FV1000-MPE

microscope multiphotonThis microscope has a confocal and a multiphoton component. 


 

Multiphoton:

Excitation Lines (Lasers): Multiphoton laser: 690-1040 nm (Spectra Physics Mai Tai-DeepSee)

Fixed Fluorescence Channels: Second Harmonics, CFP, GFP, mcherry

Image Detection: Point scanning with 4 PMT’s  

 

Confocal:

Confocal lasers: 405, 458, 488, 515, 559, 635 nm 

Confocal Emission:  Filter wheel (variable channels) 

Image Detection:  Point scanning with 3 PMT’s   

 
   

Microscope: Olympus Inverted IX81 

Objectives:   25X NA= 1.05 water  

Software: Olympus FV1000 acquisition package 

XY-Stage: Motorized 

Epi-Fluorescence Filters: DAPI, eGFP, Cy3 

Environmental Control: Heated Chamber

 Additional Features: Multi-channel fluorescence in confocal and multiphoton mode; Z-stacking; Time-lapse, with multiple stage positions; Photoactivation, FRET, FRAP, Mosaic
 

 

 


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Price 211A

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