Analytical Imaging Facility

Multiphoton Olympus FV1000-MPE

microscope multiphotonThis microscope has a confocal and a multiphoton component. 

 Microscope: Olympus Inverted IX81 

Objectives:   25X NA= 1.05 water  


Excitation Lines (Lasers): Multiphoton laser: 690-1040nm (Spectra Physics Mai Tai-DeepSee)

Fixed Fluorescence Channels: Second Harmonics, CFP, GFP, Mcherry

Image Detection: Point scanning with 4 PMT’s  



Confocal lasers: 405nm, 458nm, 488nm, 515nm, 559nm, 635nm 

Confocal Emission:  Filter wheel (variable channels) 

Image Detection:  Point scanning with 3 PMT’s   



Software: Olympus FV1000 acquisition package 

XY-Stage: Motorized 

Epi-Fluorescence Filters: DAPI, eGFP, Cy3 

Environmental Control: Heated Chamber

 Additional Features: Z-stacking; Time-lapse; Multiple point visiting; Photoactivation, FRET, FRAP, Mosaic



Price 211A

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