Light Microscopy

Mouse hypothalamus, CSF-1R promoter activity showing co-incidence. 

Erblich B. et.al. 2011. PLoS ONE. 10.1371 

Imaging Applications:

• Live cells, fixed cells, and tissue 
• Multi-channel fluorescence
• Z-stacking
• Time-lapse, with multiple stage positions
• Environmental control
• FRET
• Deconvolution
• Microinjection
• Mosaic
• Structural illumination
• Spectral karyotype, FISH
• Correlative fluorescence and electron microscopy 

GFP Microtubules in drosophilla S2 cells.  

Image taken by Vera DesMarais. Sample provided by David Sharp.

Microscopes:

• Digital Station #1 
• Digital Station #2 
• Digital Station #6/SKY 
• Zeiss Observer  
• Zeiss Observer CLEM 
• Delta Vision Core 
• Delta Vision Personal 
• Digital Station #5 

Time-lapse of GFP-tubulin drosophila S2 cells. 

Zhang, D., et al. 2011. Nat. Cell Biol. 13:361  

Point scanning confocal: 

• Leica SP2 
• Leica SP5 

Line scanning confocal: 

• Zeiss 5Live Duo Scan 

 Spinning disk confocal: 

•  Perkin Elmer Spinning Disk 

Expression of transcription factors Emc and Da in drosophila.  

Bhattacharya, A., et al. 2011. Cell. 147:881  

Imaging Applications:

• Live cells, fixed cells, tissue, and small organisms
• Multi-channel fluorescence
• Z-stacking
• Time-lapse
• Environmental control 
• Photoactivation, FRET, FLIP, FRAP, iFRAP
• Mosaic 

CFP tumor cells and GFP macrophage.   

Wyckoff, J., et al. 2011.Cold Spring Harb Protoc. 10:1101 

Imaging Applications:

• Live cells, fixed cells, tissue, and whole animal intra-vital imaging
• Multi-channel fluorescence in confocal and multiphoton mode
• Z-stacking
• Time-lapse, with multiple stage positions
• Environmental control
• Photoactivation, FRET, FRAP
• Mosaic

 Microscope:

• Multiphoton Olympus FV1000-MPE 

 Oil red O stained Axl -/- mouse spinal cord section.  

Weinger, JG. et al. 2011. J Neuroinflammation. 15;8:49.   

Imaging Applications: 

• Fixed cells, tissue, and histology slides
• Color imaging
• Darkfield, Brightfield, DIC, Phase, Polarized light

 

H&E staining of uterine gland and stroma of human origin. 

Polotsky, D. et al. 2009.Hum Reprod. 24:1960.  

Microscopes:

• Zeiss Axioskop II 
• Zeiss Observer  
• Zeiss Observer CLEM 

 

Mouse embryo. 

Image taken by Hillary Guzik. Sample provided by Juraj Kokavec. 

Imaging Applications: 

• Small animals, embryos, organs, tissue, and culture dishes 
• Color imaging

Microscopes: 

• Zeiss STEMI  
• Nikon Macro Photography