Analytical Imaging Facility

Electron Microscopy

The Analytical Imaging Facility provides complete sample preparation utilizing a broad spectrum of techniques for examination by Transmission Electron Microscopy (TEM) or Scanning Electron Microscopy (SEM). Whether your interests lie in standard morphology or immunohistochemistry our staff will design protocols that will facilitate your experiments and optimize your results. Once the samples are processed, users can either work on the microscopes with one of our staff or independently (following training). Please see or contact one of our staff to plan a future experiment.

Transmission Electron Microscopes

Equipment EM TEM 

Dystrophic axons and axonal spheroids in PBS- and rhGas6-treated mice. 

Tsiperson, V. et al. 2012. PLoS One. 5:e15748. 

 

    Imaging Applications: 

    • Cellular structure
    • Electron tomography
    • Single particle analysis
    • Macromolecular localization
    • Host-pathogen interaction
    • Tissue pathology
 

 

 
 

Scanning Electron Microcope

Equipment EM SEM 

E. coli BL21(DE3) cells expressing EfsQnr. 

Hegde, S. et al. 2010. Antimicrob Agents Chemother. 55:110. 

 

    Imaging Applications: 

    • Surface imaging
    • Macromolecular localization
    • Host-pathogen interactions
    • Elemental analysis
    • 3-D reconstruction
    • Correlative electron microscopy
 

 

 
 
 

Specimen Preparation for Electron Microscopy

Equipment specimen prep 

    Sample Preperation Applications: 

    • Embedding
    • Thin Sectioning
    • Negative Staining
    • Freeze Fracture
    • Immunogold Labeling
    • Cryoultramicrotomy
    • Tokyasu immunogold labeling
    • Critical Point Drying
    • Sputter Coating
    • Slam Freeze Cryofixation
    • High Resolution Rotary Shadowing
    • High Pressure Freezing
    • Freeze Substitution
    • Low Temperature Embedding
    • Plunge Freezing
 

 

 
 

 

All projects must be approved by Frank Macaluso in advance. Experimental design, special issues (such as dealing with pathogens), and scheduling must  also be arranged in advance. 

 
 

TEM Image Gallery
SEM Image Gallery 

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