Analytical Imaging Facility

Specimen preparation equipment

The staff of the AIF offers full service sample preparation for many standard and state of the art EM techniques. These include: 

 denton 

  • Embedding utilizing either epoxy or acrylic resins at ambient or low temperatures 
  • Thin Sectioning with a Leica EMUC7, Leica UCT or Reichert Ultracut E Ultramicrotome  
  • Negative Staining of viruses and bacteria  
  • Freeze Fracture using a Cressington CFE-50 Freeze Etch Unit 
  • Immunogold Labeling following pre or post embedding protocols 
  • Cryoultramicrotomy utilizing a Leica UCT cryoultramicrotome 
  • Tokyasu immunogold labeling of ultrathin cryo sections  
  • Critical Point Drying with a Tousimis Samdri 790 Critical Point Dryer 
  • Sputter Coating using a EMS 150TES coating unit 
  • Slam Freeze Cryofixation using a Life Cell CF100 Slam Freezer 
  • High Resolution Rotary Shadowing in a Cressington CFE-50  
  • High Pressure Freezing using a Bal-Tec HPM-010 High Pressure Freezing Machine  
  • Freeze Substitution and Low Temperature Embedding in a RMC FS-7500 Freeze Substitution 
Unit  
  • Plunge Freezing of macromolecules for cryoEM using a FEI Vitrobot 

 

 

 

 



 Electron Microscopes
Electron Microscopy Preparation

 

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