Analytical Imaging Facility

Light Microscopy

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 Widefield Fluorescence Microscopes 

 

Equipment light fluorescence pollard lab 

Mouse hypothalamus, CSF-1R promoter activity showing co-incidence. 

Erblich B. et.al. 2011. PLoS ONE. 10.1371 

 

    Imaging Applications:

    • Live cells, fixed cells, and tissue 
    • Multi-channel fluorescence
    • Z-stacking
    • Time-lapse, with multiple stage positions
    • Environmental control
    • FRET
    • Deconvolution
    • Microinjection
    • Mosaic
    • Structural illumination
    • Spectral karyotype, FISH
    • Correlative fluorescence and electron microscopy 

 

Equipment light widefield advanced 

GFP Microtubules in drosophilla S2 cells.  

Image taken by Vera DesMarais. Sample provided by David Sharp.

 

 

 
 
 

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Laser Confocal Microscopes

Equipment light live cell confocal  

Time-lapse of GFP-tubulin drosophila S2 cells. 

Zhang, D., et al. 2011. Nat. Cell Biol. 13:361  

    

 

 

Equipment light point scanner confocal  

Expression of transcription factors Emc and Da in drosophila.  

Bhattacharya, A., et al. 2011. Cell. 147:881  


 

    Imaging Applications:

    • Live cells, fixed cells, tissue, and small organisms
    • Multi-channel fluorescence
    • Z-stacking
    • Time-lapse
    • Environmental control 
    • Photoactivation, FRET, FLIP, FRAP, iFRAP
    • Mosaic 

 

 
 
 
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Multiphoton Microscope

 

Equipment light multiphoton  

CFP tumor cells and GFP macrophage.   

Wyckoff, J., et al. 2011.Cold Spring Harb Protoc. 10:1101 

 

    Imaging Applications:

    • Live cells, fixed cells, tissue, and whole animal intra-vital imaging
    • Multi-channel fluorescence in confocal and multiphoton mode
    • Z-stacking
    • Time-lapse, with multiple stage positions
    • Environmental control
    • Photoactivation, FRET, FRAP
    • Mosaic
 

 

 

 

 

 

 

 

 

 

 

 

 
 
 
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Transmitted Light Microscopes

 

Equipment light brightfield 2  

 Oil red O stained Axl -/- mouse spinal cord section.  

Weinger, JG. et al. 2011. J Neuroinflammation. 15;8:49.   

 

    Imaging Applications: 

    • Fixed cells, tissue, and histology slides
    • Color imaging
    • Darkfield, Brightfield, DIC, Phase, Polarized light

     

 

  Equipment light BF pollard 

H&E staining of uterine gland and stroma of human origin. 

Polotsky, D. et al. 2009.Hum Reprod. 24:1960.  

 

 

 
 
 
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Macro Photography

 

Equipment light  brightfield macro 

Mouse embryo. 

Image taken by Hillary Guzik. Sample provided by Juraj Kokavec. 

    Imaging Applications: 

    • Small animals, embryos, organs, tissue, and culture dishes 
    • Color imaging
    • Fluorescence imaging
 

 

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