Signifies key resources
Current Protocols in Cytometry, available online through AECOM's library!
Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available. Updated every three months in all formats, Current Protocols in Cytometry is constantly evolving to keep pace with the very latest discoveries and developments. On average, 52% of content is new and 48% has been revised every year since initial publication.
Current Protocols in Immunology, also available online through AECOM's library!
Current Protocols in Immunology is also a "best-practices" collection that provides comprehensive coverage of immunological methods from classic to the most cutting edge: the most sophisticated protocols in immunology today. Updated every three months in all formats, Current Protocols in Immunology is constantly evolving to keep pace with the very latest discoveries and developments. Since the initial publication of the work, 770 pages of content is new (43%) and revised (57%) on average every year! Current Protocols in Immunology covers the latest in antibody detection and preparation, molecular immunology, innate immunity, tumor immunology, engineering immune molecules and receptors, and more; as well as animal models for analyses; offers valuable reference information in five extensive appendices, including commonly used reagents, equipment and techniques; and a full appendix devoted to the CD system of leukocyte surface molecules.
Antibody Cross-Reactivity Resources:
Keith Bahjat
NIH Nonhuman Primate Reagent Resource - hosted by Beth Israel Deaconess Medical Center
Washington State University College of Veterinary Medicine
Apoptosis:
Apoptosis - University of Texas MD Anderson Cancer Center
Apoptosis manual
CellDeath.de
Caspases - includes many links to important sites.
Multiparametric Analysis of Apoptosis by Flow and Image Cytometry - by William Telford, Akira Komoriya and Beverly Packard
Becton Dickinson Procedures
Cell Cycle basics - from University College London
Cell Cycle protocols and analysis - University of Texas MD Anderson Cancer Center
CellQuest Pro User's Guide from BD
CellQuest User's Guide from BD
Compensation - from Mario Roederer, PhD
Conjugation of monoclonal antibodies - also by Mario Roederer, PhD
Cytometry Techniques and Protocols (Purdue)
Diva Quick Start Guide - from Becton Dickinson
Flow Cytometry on the Web - Salk Institute - We're rated 5th!!!
FlowJo analysis software:
FlowJo Basic Tutorial - hard copy
FlowJo Basic Tutorial - video
FlowJo Cell Cycle Tutorial - hard copy
FlowJo Compensation Tutorial - hard copy
FlowJo Compensation Tutorial - video 1
FlowJo Compensation Tutorial - video 2
FlowJo Compensation Tutorial - video 3
Compensation panel discussion - video
FlowJo Kinetics Tutorial - hard copy
FlowJo Software Reference Manuals:
FlowJo for Macintosh Reference Manual
FlowJo for Windows Reference Manual
Fluorescence Tutorials - by Invitrogen
Fluorescence Spectrum Viewers:
Becton Dickinson
Invitrogen
G-force calculator for your centrifuge - it's the G-force that's important, not the RPMs!
Intracellular cytokine protocols - by Joost Schuitemaker
ModFit LT 3.0 software:
Auto analysis movie tutorial
Manual analysis movie tutorial
Proliferation Wizard movie tutorial
Synchronization Wizard movie tutorial - used to analyze data with perturbed populations
"Practical Flow Cytometry" 4th Ed. (published by John Wiley & Sons), by Howard Shapiro, can be accessed free of charge! While you can't print the materials, you can read whatever you want. This is a great gift to the community by Molecular Probes detection technologies from Invitrogen. Alternatively, a short form will allow you direct access to the registration page (your name and email are required).
Protocols on the web including flow cytometry
Purdue flow cytometry
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques - by Bill Telford, Louis King and Pamela Fraker
Technical Center for those using secondary and tertiary antibodies: http://www.jacksonimmuno.com/home.asp and click the 'Technical Center' tab.
Tissue Dissociation Guide - by Worthington Biochemical Corporation
Titering Antibodies - by Carlton and Sigrid Stewart
Which Statistic When?
WinMDI Tutorial - by Gérald Gergori, Kathy Ragheb and J. Paul Robinson
VENDOR WEB SITES
Becton Dickinson/Pharmingen (flow cytometers, antibodies, etc.)
Beckman Coulter (flow cytometers, antibodies, etc.)
DakoCytomation (flow cytometers, antibodies, etc.)
Partec (flow cytometers, antibodies, etc.)
Miltenyi Biotec (magnetic antibody bead separation instrumentation and associated antibodies)
Invitrogen (Molecular Probes/Caltag/Qdots/CloneTech)
Jackson ImmunoResearch Laboratories (quality secondary antibodies)
eBioscience
Sigma-Aldrich
Fisher Scientific
Bioss