Postdoctoral Studies

Postdoctoral Program

Job Title:

Postdoctoral Position in Laboratory of David C. Spray, PhD

Job Description:

The major interest of my laboratory is in understanding the roles of the gap junction superfamily (connexins and pannexins) in intercellular communication in the normal and pathological nervous system.  There are currently three main projects in my lab:

1)      Biophysical properties of gap junction channels and their variants.  Using electrophysiology (whole cell voltage and patch clamp techniques), we are characterizing properties of mouse and human connexins, pannexins and their variants using exogenous expression in otherwise null cell lines;

2)      Gap junction control of blood-brain-barrier (BBB) tightness.  The neurovascular unit consisting of endothelial cells and astrocytes is critical in maintenance of the blood- BBB, and gap junctions in the perivascular endfeet are essential in this process.  We are studying how gap junctions in astrocytes affect the other proteins in astrocyte endfeet using Fluorescence Recovery After Photobleaching (FRAP) and super-resolution microscopy, how astrocyte control BBB permeability using coculture in tissue culture inserts and how gap junction and other endfoot proteins affect BBB in vivo using transgenic mice; 

3)      Signaling between glia and neurons in sensory ganglia, and role of this signaling in chronic pain.  For this, we are using several mouse pain models applied to mice with the genetically encoded calcium indicator GCaMP targeted to glia and to neurons and mice with manipulated expression of gap junctions and pannexin in specific cell populations.  Techniques include behavioral analysis, intracellular Ca2+ imaging of neurons and glia in sensory ganglia, patch clamp and whole animal electrophysiology, confocal and super resolution imaging of cells and tissue sections, and common methods of biochemical and molecular analysis. 

To apply, please email your CV, a cover letter summarizing your previous research experience, current research interests and future goals, along with the contact information of three references to the address below.

The Albert Einstein College of Medicine is an equal opportunity employer committed to workforce diversity.

 

Contact: 

David C. Spray, PhD
Professor of Dominick P. Purpura Department of Neuroscience and Department of Medicine (Cardiology)
840 Kennedy Center, 410 Pelham Parkway So, Bronx, NY 10461
Phone:  (718) 430-2537
email:  david.spray@einstein.yu.edu 

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