Techniques:

  • Isolation and manipulation of pre- and post-implantation embryo
  • In vitro fertilization
  • Embryonic stem cell culture
  • Embryo culture
  • Blastocyst microinjection
  • Pronuclear microinjection of DNA
  • Tetraploid aggregations
  • Mutagenesis screens
  • Construct design
  • Conditional knockout strategies
  • Regulated transgenic systems
  • "Reporter" mice (ROSA26, GFP)
  • Transfections and screening strategies
  • Embryo cryopreservation
  • Humane mouse handling and colony maintenance
  • In situ hybridization and immunohistochemical techniques
  • Mouse genomics
  • DNA microarrays

Please check back frequently to see additional techniques.

Last Updated: March 24, 2004

Course reading list:

Joyner, A.L., 1993
Gene Targeting, A Practical Approach
IRL Press

Rugh, R., 1990
The Mouse, It’s Reproduction and Development
Oxford University Press

Copp, A.J. and Cockroft, D.L., 1990
Postimplantation Mammalian Embryos, A Practical Approach
IRL Press

Kaufman, M.H., 1992
The Atlas of Mouse Development
Academic Press

Kaufman, M.H., and Bard, J.B.L., 1999
The Anatomical Basis of Mouse Development
Academic Press

Hogan, B., Costantini, F., Lacy, E., 1986
Manipulating the Mouse Embryo, A Laboratory Manual
Cold Spring Harbor Laboratory

Wassarman, P.M., DePamphilis, M.L., 1993
Guide to Techniques in Mouse Development
Academic Press