Laboratory of Robert H. Singer, Ph.D.

Engineering & Operations

Shailesh M Shenoy
Shailesh M Shenoy
Senior Associate of Anatomy & Structural Biology
Director of Engineering & Operations, Gruss Lipper Biophotonics Center
Director of Singer Laboratory Operations

shailesh.shenoy@einstein.yu.edu
Golding Room 604A, +1.718.430.3232, faculty profile
Publications:
26.
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β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration.
Katz ZB, Wells AL, Park HY, Wu B, Shenoy SM, Singer RH.
Genes Dev 26(17):1885-1890 (2012 September 1). PMCID: PMC3435492
25.
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Single-mRNA counting using fluorescent in situ hybridization in budding yeast.
Trcek T, Chao JA, Larson DR, Park HY, Zenklusen D, Shenoy SM, Singer RH.
Nat Protoc 7(2):408-419 (2012 February).
24.
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Altered dynamics of intestinal cell maturation in Apc1638N/+ mice.
Wang D, Pezo RC, Corner G, Sison C, Lesser ML, Shenoy SM, Mariadason JM, Singer RH, Augenlicht LH.
Cancer Res 70(13):5348-5357 (2010 July 1). PMCID: PMC2906237
23.

Visualization and Quantification of Single RNA Molecules in Living Cells.
Shav-Tal Y, Shenoy SM, Singer RH.
In Live Cell Imaging: A Laboratory Manual, Second Edition, eds Goldman RD, Swedlow JR, Spector DL, 697-712. Cold Spring Harbor Laboratory Press (2009 December 30).
22.
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Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells.
Maier S, Daroqui MC, Scherer S, Roepcke S, Velcich A, Shenoy SM, Singer RH, Augenlicht LH.
J Cell Physiol 218(3):638-642 (2009 March). PMCID: PMC2763190
21.
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Calibrating excitation light fluxes for quantitative light microscopy in cell biology.
Grünwald D, Shenoy SM, Burke S, Singer RH.
Nat Protoc 3(11):1809-1814 (2008 November). PMCID: PMC3109976
20.
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A role for VICKZ proteins in the progression of colorectal carcinomas: Regulating lamellipodia formation.
Vainer G, Vainer-Mossel E, Pikarsky A, Shenoy SM, Oberman F, Yeffet A, Singer RH, Pikarsky E, Yisraeli JK.
J Pathol 215(4):445-456 (2008 August). PMCID: PMC3148580
19.
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The spatial order of transcription in mammalian cells.
Levsky JM, Shenoy SM, Chubb JR, Hall CB, Capodieci P, Singer RH.
J Cell Biochem 102(3):609-617 (2007 October 15).
18.
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In vivo dynamics of RNA polymerase II transcription.
Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, Singer RH.
Nat Struct Mol Biol 14(9):796-806 (2007 September).
Comment in Nat Struct Mol Biol. 2007 Sep;14(9):788-90.
17.
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Visualization of mRNA translation in living cells.
Rodriguez AJ, Shenoy SM, Singer RH, Condeelis J.
J Cell Biol 175(1):67-76 (2006 October 9). PMCID: PMC2064499
16.
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Transcriptional pulsing of a developmental gene.
Chubb JR, Trcek T, Shenoy SM, Singer RH.
Curr Biol 16(10):1018-1025 (2006 May 23).
Comment in Curr Biol. 2006 May 23;16(10):R371-3.
15.
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Gene expression profiling in single cells within tissue.
Capodieci P, Donovan M, Buchinsky H, Jeffers Y, Cordon-Cardo C, Gerald W, Edelson J, Shenoy SM, Singer RH.
Nat Methods 2(9):663-665 (2005 September). PMCID: PMC1976602
14.
PubMed citation for publication #14
Imaging of Single mRNAs in the Cytoplasm of Living Cells.
Shav-Tal Y, Shenoy SM, Singer RH.
In Live Cell Imaging: A Laboratory Manual, eds Goldman RD, Spector DL, 603-615. Cold Spring Harbor Laboratory Press (2004 October).
13.
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Dynamics of Single mRNPs in Nuclei of Living Cells.
Shav-Tal Y, Darzacq X, Shenoy SM, Fusco D, Janicki SM, Spector DL, Singer RH.
Science 304(5678):1797-1800 (2004 June 18).
12.
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Role of the parafusin orthologue, PRP1, in microneme exocytosis and cell invasion in Toxoplasma gondii.
Matthiesen SH, Shenoy SM, Kim K, Singer RH, Satir BH.
Cell Microbiol 5(9):613-624 (2003 September).
11.
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Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization.
Zhang HL, Pan F, Hong D, Shenoy SM, Singer RH, Bassell GJ.
J Neurosci 23(16):6627-6637 (2003 July 23).
10.
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Asymmetric Distribution of Nuclear Pore Complexes and the Cytoplasmic Localization of ß2-Tubulin mRNA in Chlamydomonas reinhardtii.
Colón-Ramos DA, Salisbury JL, Sanders MA, Shenoy SM, Singer RH, Garciá-Blanco MA.
Dev Cell 4(6):941-952 (2003 June).
9.
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Activity-Dependent Trafficking and Dynamic Localization of Zipcode Binding Protein 1 and beta-Actin mRNA in Dendrites and Spines of Hippocampal Neurons.
Tiruchinapalli DM, Oleynikov Y, Kelic S, Shenoy SM, Hartley A, Stanton PK, Singer RH, Bassell GJ.
J Neurosci 23(8):3251-3261 (2003 April 15).
8.
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Single mRNA molecules demonstrate probabilistic movement in living mammalian cells.
Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, Bertrand E.
Curr Biol 13(2):161-167 (2003 January 21).
7.
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Novel detection and differential utilization of a c-myc transcriptional block in colon cancer chemoprevention.
Wilson AJ, Velcich A, Arango D, Kurland AR, Shenoy SM, Pezo RC, Levsky JM, Singer RH, Augenlicht LH.
Cancer Res 62(21):6006-6010 (2002 November 1).
6.
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Single-cell gene expression profiling.
Levsky JM, Shenoy SM, Pezo RC, Singer RH.
Science 297(5582):836-840 (2002 August 2).
5.
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A parafusin-related Toxoplasma protein in Ca2+-regulated secretory organelles.
Matthiesen SH, Shenoy SM, Kim K, Singer RH, Satir BH.
Eur J Cell Biol 80(12):775-783 (2001 December).
4.
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Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility.
Zhang HL, Eom T, Oleynikov Y, Shenoy SM, Liebelt DA, Dictenberg JB, Singer RH, Bassell GJ.
Neuron 31(2):261-275 (2001 August 2).
3.
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Localization of ASH1 mRNA Particles in Living Yeast.
Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM.
Mol Cell 2(4):437-445 (1998 October).
2.
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Quantitative digital analysis of diffuse and concentrated nuclear distributions of nascent transcripts, SC35 and poly(A).
Fay FS, Taneja KL, Shenoy S, Lifshitz L, Singer RH.
Exp Cell Res 231(1):27-37 (1997 February 25).
1.
PubMed citation for publication #1
Human immunodeficiency virus detection in lymph nodes by image analysis of in situ hybridization: basis of a diagnostic test for cadaveric donors.
Mathijs JM, Shenoy SM, Singer RH.
Transplant Proc 28(5):2945-2949 (1996 October).
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