Jeffrey Segall's Lab

Jeffrey E. Segall


Harvard College, Cambridge, MA B.A. 1979 Biology and Physics
California Inst. Technology, CA Ph.D. 1984 Biophysics


Post-graduate Training:

1984-1989 Postdoctoral Fellow, Department of Cell Biology
Max Planck Institute for Biochemistry, W. Germany


Professional Employment:

1989-1995 Assistant Professor, Department of Anatomy and Structural Biology
Albert Einstein College of Medicine, Bronx, NY
1995-2000 Associate Professor, Department of Anatomy and Structural Biology
Albert Einstein College of Medicine, Bronx, NY
2000- present Professor, Department of Anatomy and Structural Biology
Albert Einstein College of Medicine, Bronx, NY


Professional Society Membership:

Since 1993 American Society of Cell Biology
Since 1997 American Association for Cancer Research
Since 1998 American Association for the Advancement of Science

Jeff Segall 

Awards and Honors:

1979-1982 National Science Foundation Predoctoral Fellowship
1981-1983 Arthur McCallum Fellowship
1984-1985 Max Planck Society Fellowship
1985-1986 NATO Postdoctoral Fellowship
1987 EMBO Short-term Fellowship
1991 Mellam Family Foundation Investigator Award, American Heart Association, NYC Affiliate
1995 Established Scientist Award, American Heart Association, NYC Affiliate
2000 Vice-Chair for Gordon Conference on Sensory Transduction inMicroorganisms for 2000
2002 Chair for Gordon Conference on Sensory Transduction in Microorganisms for 2002
2005 Vice-Chair for Gordon Conference on Gradient Sensing and Directed Cell Migration 2005
2007 Chair for Gordon Conference on Gradient Sensing and Directed Cell Migration 2007
2008 Betty and Sheldon Feinberg Senior Faculty Scholar in Cancer Research



A. Original Communications in Reviewed Journals

  1. Chiang, P.K., Guranowski, A. and J.E. Segall (1981) Irreversible Inhibition of S-Adenosylhomocysteine Hydrolase by Nucleoside Analogs. Arch. Bioc. Bioph. 207:175:184.
  2. Segall, J.E., Manson, M.D. and H.C. Berg. (1982) Signal Processing Times in Bacterial Chemotaxis. Nature 296:855-857.
  3. Block, S.M., Segall, J.E. and H.C. Berg. (1982) Impulse Responses in Bacterial Chemotaxis. Cell 31:215-226.
  4. Segall, J.E., Ishihara, A. and H.C. Berg. (1985) Chemotactic Signaling in Filamentous Cells of Escherichia coli. J. Bacter. 161:51-59.
  5. Segall, J.E., Block, S.M, and H.C. Berg. (1986) Temporal Comparisons in Bacterial Chemotaxis. Proc. Natl. Acad. Sci. USA 83:8987-8991.
  6. Segall, J.E., Fisher, P.R. and G. Gerisch. (1987) Selection of Chemotaxis Mutants of Dictyostelium discoideum. J. Cell Biol. 104:151-161.
  7. Segall, J.E.. (1988) Analysis of Responses of Dictyostelium discoideum Amoebae to Chemoattractant Concentration Changes. J. Muscle Res. Cell Mot. 9:481-490.
  8. Segall, J.E., Bominaar, A., Wallraff, E. and R. de Wit. (1988) Analysis of a Dictyostelium Chemotaxis Mutant with Altered Chemoattractant Binding. J. Cell Sci. 91:479-489.
  9. Andre, E., Brink, M., Gerisch, G., Isenberg, G., Noegel, A., Schleicher, M., Segall, J. E., and E. A. Wallraff. (1989) A Dictyostelium Mutant Deficient in Severin, an F-actin Fragmenting Protein, Shows Normal Motility and Chemotaxis. J. Cell Biol. 108:985-995.
  10. Brinke, J., Gerisch, G., Isenberg, G., Noegel, A.A., Segall, J.E., Wallraff, E., and M. Schleicher. (1990) A Dictyostelium Mutant lacking an F-actin Crosslinking Protein, the 120 kD Gelation Factor. J. Cell Biol. 111:1477-1489.
  11. Barisic, K., Mollner, S., Noegel, A. A., Gerisch, G., and J. E. Segall. (1991) cDNA Sequence of Cyclophilin from Dictyostelium discoideum. Developmental Genetics, 12:50-53.
  12. Segall, J.E. (1992) Behavioral responses of streamer F mutants of Dictyostelium discoideum: effects of cyclic GMP on cell motility. J. Cell Sci., 101:589-597. Segall, J.E. and G. Gerisch. (1989) Genetic Approaches to Cytoskeleton Function and the Control of Cell Motility. Ann. Rev. in Cell Biology: Cytoplasm and Cell Motility. 1:44-50.
  13. Segall, J.E. (1993) Polarization of yeast cells in spatial gradients of mating factor. Proc. Natl. Acad. Sci. 90:8332-8336.
  14. Segall, J.E., Kuspa, A., Shaulsky, G., Ecke, M., Maeda, M., Gaskins, C., Firtel, R.A., and W.G. Loomis. (1995) A MAP kinase necessary for receptor-mediated activation of adenylate cyclase in Dictyostelium. J. Cell Biol. 128:405-413.
  15. Segall, J.E., Tyrech, S., Boselli, L., W., Masseling, S., Chan, A., Helft, J, Jones, J. and J. Condeelis. (1996) EGF stimulates lamellipod extension and chemotaxis in metastatic mammary adenocarcinoma cells by an actin-dependent mechanism. Clin. Exp. Met. 14:61-72.
  16. Gaskins, C., Clark, A.M., Aubry, L., Segall, J.E., Firtel, R.A. (1996) The Dictyostelium MAP kinase ERK2 regulates multiple, independent developmental pathways. Genes and Dev. 10:118-128.
  17. Knetsch, M.L.W., Epskamp, S.J.P., Schenk, P.W., Segall, J.E., and B.E. Snaar-Jagalska. (1996) Both G-protein and ras-dependent activation of extracellular signal regulated kinase 2 is antagonized by intracellular cAMP in Dictyostelium discoideum. EMBO J. 15:3361-3368.
  18. Leberer, E., Wu, C., Leeuw, T., Fourest-Lieuvin, A., Segall, J.E., and D. Y. Thomas (1996) Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J. 16:83-97.
  19. Edmonds, B.T., Wyckoff, J., Yeung, Y.-G., Wang, Y., Stanley, E.R., Jones, J., Segall, J.E., and J. Condeelis (1996) Elongation factor-1alpha is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma. J. Cell Science 109:2705-2714.
  20. Chan, A., Raft, S., Bailly, M., Wyckoff, J., Segall, J. and Condeelis, J. (1998) EGF stimulates an increase in actin nucleation and filament number at the leading edge of the lamellipod in mammary adenocarcinoma cells. J. Cell Sci. 111:199-211.
  21. Wang, Y., Liu, J. and J.E. Segall (1998) MAP kinase function in amoeboid chemotaxis. J. Cell Sci. 111:373-383.
  22. Wang, Y. and J.E. Segall (1998) The Dictyostelium MAP kinase DdERK2 functions as a cytosolic protein in complexes with its substrates in chemotactic signal transduction. Biochem. Biophys. Res. Comm. 244:149-155.
  23. Bailly, M., Yan, L., Whitesides, G.M., Condeelis, J.S., and J.E. Segall (1998) Regulation of protrusion shape and adhesion to the substratum during chemotactic responses of mammalian carcinoma cells. Exp. Cell Res. 241:285-299.
  24. Farina, K., Wyckoff, J., Rivera, J., Lee, H., Segall, J., Condeelis, J. and Jones, J. (1998) Cell motility of tumor cells visualized in living intact primary tumors using green fluorescent protein. Cancer Res. 58:2528-2532.
  25. Wyckoff, J.B., Insel, L., Khazaie, K., Lichtner, R.B., Condeelis, J.S. and J.E. Segall (1998) Suppression of ruffling by EGF in chemotactic cells. Exp. Cell Res. 242:100-109.
  26. Bailly, M., Macaluso, F., Cammer, M., Chan, A., Segall, J.E., and J.S. Condeelis. (1999) Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after EGF stimulation. J. Cell Biol. 145:331-345.
  27. Hill, K., Welti, S., Yu, J., Murray, J.T., Yip, S., Condeelis, J.S., Segall, J.E., Backer, J.M. (2000) Specific requirement for the p85-p110alpha PI 3-kinase during EGF-stimulated actin nucleation in breast cancer cells. J. Biol. Chem. 275:3741-3744.
  28. Chan,A.Y., Bailly,M., Zebda,N., Segall,J.E, and J.S. Condeelis (2000) Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion. J Cell Biol. 148:531-542.
  29. Wyckoff, J.B.,Jones, J.G., Condeelis, J. C., and J E. Segall (2000) A critical step in metastasis: In vivo analysis of intravasation at the primary tumor. Cancer Res. 60:2504-2511.
  30. Wyckoff, J.B., Segall, J.E. and Condeelis, J.C. (2000) The collection of the motile population of cells from a living tumor. Cancer Res. 60:5401-5404.Bailly,M. , Wyckoff,J.B., Bouzahzah, B., Hammerman,R., Sylvestre,V., Cammer,M., Pestell,R.G., and J. E. Segall (2000) Distribution of the EGF receptor in chemotaxing adenocarcinoma cells. Mol. Biol. Cell. 11:3873-3883. Hill, K.M., Huang, Y, Yip, S.-C., Yu, J., Segall, J.E., and J.M. Backer. (2001) N-terminal domains of the class IA phosphoinositide 3-kinase regulatory subunit play a role in cytoskeletal but not mitogenic signaling. J. Biol. Chem. 276:16374-16378.
  31. Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L.M., Segall, J.E., and J. Condeelis (2001) The F-actin side binding activity of the Arp 2/3 complex is essential for actin nucleation and lamellipod extension. Curr. Biol. 11: 620-625.
  32. Bouzahzah, B., Albanese, C., Ahmed, F., Pixley, F., Lisanti, M. P, Segall, J.E., Condeelis, J., Joyce, J., Minden, A, Der, C.J., Chan, A., Symons, M. and R. G. Pestell. (2001) Rho Family GTPases Regulate Mammary Epithelium Cell Growth and Metastasis Through Distinguishable Pathways. Mol. Med. 7: 816–830.
  33. Wang, W., Wyckoff, J.B., Frohlich, V.C., Oleynikov, Y., Huttelmaier, S., Zavadil, J., Cermak, L., Bottinger, E.P., Singer, R.H., White, J.G., Segall, J.E., and J.S. Condeelis. (2002) Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res. 62:6278-88.
  34. Vallier, L.G., Segall, J.E., and M. Snyder. (2002) The alpha-factor receptor C-terminus is important for mating projection formation and orientation in Saccharomyces cerevisiae. Cell Motil Cytoskeleton 53:251-66.
  35. Butty, A.C., Perrinjaquet, N., Petit, A., Jaquenoud, M., Segall, J.E., Hofmann, K., Zwahlen, C., and M. Peter. (2002) A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. EMBO J 21:1565-76.
  36. Ahmed F, Wyckoff J, Lin EY, Wang W, Wang Y, Hennighausen L, Miyazaki J, Jones J, Pollard JW, Condeelis JS, Segall JE. (2002) GFP Expression in the Mammary Gland for Imaging of Mammary Tumor Cells in Transgenic Mice. Cancer Res. 62:7166-9.
  37. Wang W, Wyckoff JB, Wang Y, Bottinger EP, Segall JE, Condeelis JS. (2003) Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse. BMC Biotechnol. 3:13-25.
  38. Kempiak, S., Yip, J.-L., Backer, J., and J. E. Segall (2003) Local signaling by the EGF receptor. J. Cell Biol.162:781-787.
  39. Wyckoff J, Wang W, Lin EY, Wang Y, Pixley F, Stanley ER, Graf T, Pollard JW, Segall J, Condeelis J (2004 ) A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res. 64:7022-9.
  40. Wang W, Goswami S, Lapidus K, Wells AL, Wyckoff JB, Sahai E, Singer RH, Segall JE, Condeelis JS. (2004) Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors. Cancer Res. 64:8585-94.
  41. Kempiak SJ, Yamaguchi H, Sarmiento C, Sidani M, Ghosh M, Eddy RJ, Desmarais V, Way M, Condeelis JS, Segall JE (2005) J. Biol. Chem. 280:5836-5842.
  42. Goswami S, Sahai E, Wyckoff J, Cox D, Pixley F, Stanley ER, Segall JE, Condeelis, J (2005) Macrophages promote the invasion of carcinoma cells in vitro via a paracrine loop. Cancer Res. 65:5278-83.
  43. Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki H, Takenawa T, Condeelis J. (2005) Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 168(3):441-52.
  44. Sahai E, Wyckoff J, Philippar U, Segall JE, Gertler F, Condeelis J. (2005) Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy. BMC Biotechnol. 5:14-22.
  45. Soon L, Mouneimne G, Segall J, Wyckoff J, Condeelis J. (2005) Description and characterization of a chamber for viewing and quantifying cancer cell chemotaxis. Cell Motil Cytoskeleton. 62(1):27-34.
  46. Xue C, Wyckoff J, Liang F,Sidani M, Violini S, Tsai K-L, Zhang Z-Y, Sahai E, Condeelis J, Segall JE (2006) EGF receptor overexpression results in increased tumor cell motility in vivo coordinately with enhanced intravasation and metastasis. Cancer Res. 66:192-7
  47. Xue C, Liang F, Mahmood R, Vuolo M, Wyckoff J, Qian H, TsaiK-L, Kim M, Locker J, Zhang Z-Y, Segall JE (2006 ) ErbB3-dependent motility and intravasation in breast cancer metastasis. Cancer Res. 66: 1418-1426.
  48. Chen S, Segall JE (2006) EppA, a putative substrate of DdERK2, regulates cyclic AMP relay and chemotaxis in Dictyostelium discoideum. Eukaryot Cell. 5:1136-46.
  49. Wyckoff JB, Wang Y, Lin EY, Li JF, Goswami S, Stanley ER, Segall JE, Pollard JW, Condeelis J. (2007) Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors. Cancer Res. 67:2649-56.
  50. Wang W, Wyckoff JB, Goswami S, Wang Y, Sidani M, Segall JE, Condeelis JS. (2007) Coordinated regulation of pathways for enhanced cell motility and chemotaxis is conserved in rat and mouse mammary tumors. Cancer Res. 67:3505-11.
  51. Pu J, McCaig CD, Cao L, Zhao Z, Segall JE, Zhao M. (2007) EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells. J Cell Sci. 120 :3395-403.
  52. Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha VV, Condeelis J, Segall JE, van Rheenen J (2008) Intravital imaging of metastasis through an orthotopic Mammary Imaging Window. Nature Methods. Nature Methods. 5:1019-21
  53. Kedrin D, Wyckoff JB, Hynes NE, Arteaga CL, Segall JE (in press) ERBB1 and ERBB2 have distinct functions in tumor cell invasion and intravasation. Clin. Cancer Res.
  54. Hernandez L, Smirnova T, Kedrin D, Wyckoff J, Stanley ER, Cox D, Muller WJ, Pollard JW, van Rooijen N, Segall JE (in press) EGF/CSF-1 Induced Breast Cancer Cell Invasion is Triggered by Heregulin Beta 1 and CXCL12. Cancer Res.




B. Books, Chapters in Books, and Review Articles

  1. Segall, J.E. and G. Gerisch (1989) Genetic approaches to cytoskeleton function and the control of cell motility. Curr. Op. Cell Biol.: Cytoplasm and Cell Motility. 1:44-50.
  2. Gerisch, G., Segall, J.E. and E. Wallraff. (1989) Isolation and Behavioral Analysis of Mutants Defective in Cytoskeletal Proteins. Cell Motility and the Cytoskeleton. 14:75-79.
  3. Segall, J. E. (1990) Mutational Studies of Amoeboid Chemotaxis using Dictyostelium discoideum. In: Biology of the Chemotactic Response, (J. Armitage and J. Lackie, eds.) Cambridge University Press, Cambridge, pp 241--272.
  4. Jones, J., Segall, J.E. and J. Condeelis. (1991) Molecular analysis of amoeboid chemotaxids: parallel observation in amoeboid phagocytes and metastatic tumor cells. In Cytokines and Cell Motility (I. Goldberg and E. Rosen, eds.) Birkhauser Verlag, New York.
  5. Manson, M.D., and J.E. Segall (1992) Chemotaxis. In The Encyclopedia of Microbiology (J. Lederberg, ed.) Academic Press, San Diego.
  6. Condeelis, J., Jones, J., and J. E. Segall (1992) Chemotaxis of metastatic tumor cells: clues to mechanisms from the Dictyostelium paradigm. Cancer and Metastasis Reviews 11:55-68.
  7. Mann, S. P. Devreotes, S. Eliott, K. Jermyn, A. Kuspa, M. Fechheimer, R. Furukawa, C. Parent, J. Segall, G. Shaulsky, P. Vardy, J. Williams, and R. Firtel. (1994) Cell biological, molecular genetic, and biochemical methods to examine Dictyostelium. In: "Cell Biology: A Laboratory Handbook" (ed J. Celis), Academic Press 1:412-451.
  8. Cammer, M., Wyckoff, J. and J.E. Segall (1997) Computer-assisted analysis of single cell motility. In: Basic Cell Culture Protocols (eds. J.M. Walker and J.W. Pollard), Methods in Molecular Biology, 75:459-470.
  9. Zigmond, S.H., Foxman, E.F. and J.E. Segall (1998) Chemotaxis Assays for Eukaryotic Cells. In: Current Protocols in Cell Biology (eds) Bonifacino, J.S., et al. New York, John Wiley & Sons. (in press).
  10. Bailly, M. Condeelis, J. and Segall, J.E. (1998) Chemoattractant-induced lamellipod extension. Microscopy Research and Technique. 43:433-443
  11. Segall, J.E. (1999) Cell Polarization: Chemotaxis gets cracking. Current Biology 9:R46-R48.
  12. Pestell, R.G., Albanese, C., Reutens , A.T., Segall, J.E., Lee, R.J., and Arnold, A. (1999) The cyclins and cyclin-dependent kinase inhibitors in hormonal regulation of proliferation and differentiation. Endocr Rev 20:501-34.
  13. Condeelis, J.S., Wyckoff, J., and J. E. Segall (2000) Imaging of cancer invasion and metastasis using green fluorescent protein. Eur. J. Cancer 36:1671-1680.
  14. Condeelis. J.S., Wyckoff, J.B., Bailly, M., Pestell, R., Lawrence,D., Backer, J., and Jeffrey E. Segall (2001) Lamellipodia in invasion. Sem. Canc. Biol. 11:119-128.
  15. Condeelis, J. and J.E. Segall (2003) Intravital imaging of cell movements in tumors. Nat. Rev. Canc. 3:921-930.
  16. Condeelis J, Song X, Backer J, Wyckoff J and JE Segall (2003) Chemotaxis of cancer cells during invasion and metastasis. In: Cell Motility: from molecules to organisms. (Editors: Anne Ridley, Michelle Peckham, Peter Clark) Wiley & Sons. 175-188.
  17. Kempiak, S.J. and J.E. Segall (2004) Stimulation of cells using EGF-coated magnetic beads. Sci STKE. 218:pl1.
  18. Meili, R., Richard A. Firtel, R.A. and J. E. Segall. (2004) Molecular Mechanisms of Chemotaxis in Amoebae. In: Chemotaxis (editor Michael Eisenbach) U.K., Imperial College Press. 253-294.
  19. Bozzaro, S., Fisher P.R., Loomis, W., Satir, P. and J. E. Segall (2004) Guenther Gerisch and Dictyostelium, the microbial model for ameboid motility and multicellular morphogenesis. Trends Cell Biol. 14:585-9.
  20. Wyckoff J, Segall JE, and J Condeelis (2005) “Chapter 22: Single cell imaging in animal tumors in vivo” in Live Cell Imaging: a Laboratory Manual, Eds.: D.L. Spector and R.D. Goldman. CSHL. Cold Spring Harbor, NY. Pp. 409 - 422.
  21. Wang W, Goswami S, Sahai E, Wyckoff JB, Segall JE, Condeelis JS. (2005) Tumor cells caught in the act of invading: their strategy for enhanced cell motility. Trends Cell Biol. 15:138-45.
  22. Ingman, W., Wyckoff J, Xue C, Lin EY, Wang W, Goswami S, Pollard JW, Condeelis J, and JE Segall (2005) Imaging Invasion and Metastasis In vivo. In: Cell Motility in Cancer Invasion and Metastasis (editor Alan Wells), Kluwer Academic Publishers.
  23. Condeelis J, Singer R, Segall JE (2005) The great escape: When cancer cells hijack the genes for chemotaxis and motility. Ann. Rev. Cell Dev. Biol. 21: 695-718.
  24. Sidani M, Wyckoff J, Xue C, Segall JE, Condeelis J. (2006) Probing the microenvironment of mammary tumors using multiphoton microscopy. J Mammary Gland Biol Neoplasia. 11:151-63.
  25. Wyckoff JB, Condeelis J, Segall JE (2006) Growth factor receptors – doing more than just stimulating tumor growth? BIF Futura. 21:219-223.
  26. Kedrin D, Wyckoff J, Sahai E, Condeelis J, Segall JE (2007) Imaging of tumor cell movement in vivo. Current Protocols in Cell Biology. 19.7.1 – 19.7.17.
  27. Kedrin D, van Rheenen J, Hernandez L, Condeelis J, Segall JE (2007) Cell motility and cytoskeletal regulation in invasion and metastasis. J. Mam. Gland Biol. Neoplasia. 12: 143-52.
  28. Hernandez L, Smirnova T, Wyckoff J, Condeelis J, Segall JE (in press) In vivo assay for tumor cell invasion. Methods in Molecular Biology.
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