Research Associate Professor, Department of Pathology
My research interests have been in analytical method development to solve complex clinical/biological problems. Primary focus has been on the mass spectrometry based proteomic analysis of head and neck cancer patient specimens. Generated proteome from the analysis allows evaluation of tumor aggressiveness, potential for metastasis, and predicted response to treatments providing potential prognostic biomarkers. They can guide patient treatment to minimize patient morbidity and mortality. I am evaluating the use of mass spectrometry imaging as an alternative platform for assessing clinically relevant proteins as well that would complement surgical pathologic diagnosis.
Another focus is on the characterization of intact protein and ligand binding interactions. The modes of interaction between cancer relevant proteins and their binding partners can define sites for inhibitions. Structural information gathered from the mass spectrometry analysis can help design new inhibitors which may prevent cancer progression.
Link to NIHMS profile:
1. Hishimoto A, Nomaru H, Ye K, Nishi A, Lim J, Aguilan JT, et al. Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units. Biological psychiatry. 2015.
2. Harris TM, Du P, Kawachi N, Belbin TJ, Wang Y, Schlecht NF, et al. Proteomic analysis of oral cavity squamous cell carcinoma specimens identifies patient outcome-associated proteins. Archives of pathology & laboratory medicine. 2015;139(4):494-507.
3. Carvallo L, Lopez L, Che FY, Lim J, Eugenin EA, Williams DW, et al. Buprenorphine decreases the CCL2-mediated chemotactic response of monocytes. Journal of immunology (Baltimore, Md : 1950). 2015;194(7):3246-58.
4. Tarassishin L, Lim J, Weatherly DB, Angeletti RH, Lee SC. Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. Journal of proteomics. 2014;99:152-68.
5. Lim J, Liu Z, Apontes P, Feng D, Pessin JE, Sauve AA, et al. Dual mode action of mangiferin in mouse liver under high fat diet. PloS one. 2014;9(3):e90137.
6. Wang S, Lim J, Thomas K, Yan F, Angeletti RH, Schramm VL. A complex of methylthioadenosine/S-adenosylhomocysteine nucleosidase, transition state analogue, and nucleophilic water identified by mass spectrometry. Journal of the American Chemical Society. 2012;134(3):1468-70.
7. Lim J, Menon V, Bitzer M, Miller LM, Madrid-Aliste C, Weiss LM, et al. Frozen tissue can provide reproducible proteomic results of subcellular fractionation. Analytical biochemistry. 2011;418(1):78-84.
8. Drumm JE, Mi K, Bilder P, Sun M, Lim J, Bielefeldt-Ohmann H, et al. Mycobacterium tuberculosis universal stress protein Rv2623 regulates bacillary growth by ATP-Binding: requirement for establishing chronic persistent infection. PLoS pathogens. 2009;5(5):e1000460.
9. Du P, Stolovitzky G, Horvatovich P, Bischoff R, Lim J, Suits F. A noise model for mass spectrometry based proteomics. Bioinformatics. 2008;24(8):1070-7.
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Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Ullmann Building, Room 405
Bronx, NY 10461