Associate, Department of Biochemistry
I had been focusing on the effect of high fat feeding during gestation, weaning and throughout the adulthood of mice. By using a murine model that is genetically predisposed to developing diabetes, I hope to learn more about the direct effect of a maternal high fat diet (HFD) on the offspring during gestation and early life. By examining epigenetic modifications in the setting of a high maternal high fat diet may provide an important link between the prenatal environment and increased susceptibility to adult disease.
More recently I had been working on study examining the alteration of the DNA methylation and mRNA patterns of CD3+ cord blood cells. One of the goals was to identify differences in the DNA methylation profile in purified CD3+ T-cells between IUGR and AGA term newborns. Subpopulations of CD3+ T-cells such as CD4+ and CD8+ cells play pivotal roles in the development of obesity and inflammation. By identifying distinct CD3+ methylation patterns in IUGR male infants, I tried to show how these epigenomic alterations potentially affect the T-cell lineages that are related to inflammatory changes seen in metabolic syndrome. Secondary objective is to map these methylation profiles to known metabolic pathways/genes, thereby identifying possible significant genetic elements or biomarkers that could prove useful in future research and clinical studies.
I have been currently working on developing a method for the efficient and systematic identification of the repertoire of receptor-ligand interactions relevant to human physiology, disease, and medicine using a cell-cell binding assay.
Williams, L.,* Seki, Y.,* Delahaye, F.,*Cheng, A., Fuloria, M., Einstein, F.H., Charron, M.J. 2016. DNA Hypermethylation of CD3+ T-cells from cord blood of intrauterine growth restricted humans. Diabetologia. 59(8):1714-23. (*LW, YS and DF made equal contributions).
Heyman , L., Seki, Y., Storm, P., Jones, H., Charron, M.J., Berger, K., Holm, C. 2015. Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet. J Nutr Biochem. Sep 2. pii: S0955-2863(15)00223-5. doi: 10.1016/j.jnutbio.2015.08.022.
Chi, Y., Jasmin, J., Seki, Y., Lisanti, M., Charron, M.J., Lefer, D., Schuster, V. 2015. Inhibition of the prostaglandin transporter PGT lowers blood pressure in hypertensive rats and mice. PLoS One. Jun 29;10(6):e0131735. doi: 10.1371/journal.pone.0131735.
Atkins, K*., Seki, Y*., Saha, J., Eichinger, F., Charron, M.J., Brosius, F.C. 2015. Maintenance of GLUT4 expression in smooth muscle prevents hypertension-induced changes in vascular reactivity. Physiol Rep. 3 (1), e12299. (*KA and YS made equal contributions).
Plata, M., Williams, L., Seki, Y., Hartil, K., Kaur, H., Lin, C., Fiallo, A., Glenn, A.S., Katz, E.B., Fuloria, M., Charron, M.J., Vuguin, P.M. 2014. Critical periods of increased fetal vulnerability to adverse metabolic programming by a maternal high Fat diet in CD1 mouse model. Reprod Biol Endocrinol. Aug 18;12(1):80.
Lin, C.L., Williams, L., Seki, Y., Kaur, H., Hartil, K., Fiallo,A., Glenn, A.S., Katz, E.B., Charron, M.J., Vuguin, P.M. 2014. Effects of genetics and in utero diet on murine pancreatic development. J Endocrinol 222(2):217-27.
Williams, L*., Seki, Y*., Vuguin, P.M., Charron, M.J. 2013. Animal models of in utero exposure to a high fat diet. Biochimi Biophys Acta 2013 S0925-4439 (13) 00246-9. (*LW and YS made equal contributions).
Kruse, M., Seki, Y., Du, X., Fiallo, A., Glenn, A., Singer, S., Breuhahn, K., Katz, E.B., Charron, M.J. 2013. High fat intake during pregnancy and lactation exacerbates high fat diet induced complications in male offspring in mice. Endocrinology 154(10): 3565-76.
Vuguin, P.M., Hartil, K., Kruse, M., Kaur, H., Chia-Lei, L., Scott, A.S., Fiallo, A., Patel, A., Williams, L., Seki, Y., Katz, E.B., Charron M.J. 2013. Shared effects of genetic and intrauterine environment on the development of metabolic syndrome. PloS One 17;8(5): e63021.
Seki, Y., Williams, L., Vuguin, P.M., Charron, M.J. 2012. Epigenetic programming of diabetes and obesity: animal models. Endocrinology 153, 1031–1038.
Ranalletta M, Du X, Seki Y, Glenn AS, Kruse M, Fiallo A, Estrada I, Tsao TS, Stenbit AE, Katz EB, Charron MJ. 2007. Hepatic response to restoration of GLUT4 in skeletal muscle of GLUT4 Null mice. Am J Physiol Endocrinol Metab 293, 1178-1187.
Seki, Y., Berggren, JA., Houmard, JA., Charron, M.J. 2006. Glucose transporter expression in skeletal muscle of endurance-trained individuals. Med Sci Sport Exer 38, 1088-1092.
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