Professor, Department of Biochemistry
Biology is a dynamic process. Among the myriad array of reversible association reactions that constitute life, small molecules bind to proteins, proteins self-associate and bind to other proteins and nucleic acids and nucleic acids fold and bind to each other in elaborate processing, signaling and regulatory cascades. What is common to these processes is the physical chemistry that underlies these interactions. For example, electrostatic interactions mediate both the binding of proteins to DNA and the folding of RNA. Proteins that mimic the electrostatic character of DNA may competitively regulate DNA binding by other proteins. Our laboratory seeks answers to questions related to the structure – function relationships that govern macromolecular function by combining quantitative analysis with innovative approaches.
LoPiccolo, J., Kim, S.J., Shi, Y., Wu, B., Wu, H., Chait, B.T., Singer, R.H., Sali, A., Brenowitz, M., Bresnick, A.R., Backer, J.M. (2015) Assembly and Molecular Architecture of the Phosphoinositide 3-Kinase p85α Homodimer, J Biological Chemistry 290(51), 30, 390-30,405
Shymanets, A., Prajwal, Vadas, O., Czupalla, C., LoPiccolo, J., Brenowitz, M., Ghigo, A., Hirsch, E., Krause, E., Wetzker, R., Williams, R.L., Harteneck, C., Nürnberg B. (2015) Different inhibition of Gβγ-stimulated class IB phosphoinositide 3-kinase (PI3K) variants by a monoclonal antibody. Specific function of p101 as a Gβγ-dependent regulator of PI3Kγ enzymatic activity, Biochemical J 469(1), 59 – 69
Dai, Z., Tao, Y., Liu, N., Brenowitz, M.D., Girvin, M.E. & Lai, J.R. (2015) Conditional Trimerization and Lytic Activity of HIV-1 gp41 Variants containing the Membrane-Associated Segments, Biochemistry 54(8), 1589 – 99
Leser, M., Pegan, J., El Makkaoui, M., Schlatterer, J.C., Khine, M., Law, M.& Brenowitz, M. (2015) Protein Footprinting by Pyrite Shrink-Wrap Laminate, Lab on a Chip 15(7), 1646 – 50
Liu, N., Tao, Y., Brenowitz, M.D., Girvin, M.E. & Lai, J.R. (2015) Structural and Functional Studies on the Marburg Virus GP2 Fusion Loop, Journal of Infectious Disease Suppl 2, S146 - 53
Khrapunov, S., Warren, C., Cheng, H., Berko, E., Greally, J.M. & Brenowitz, M. (2014) Unusual characteristics of MBD, the DNA binding domain of epigenetic regulatory protein MeCP2, determine its binding specificity, Biochemistry, 53(21), 3379-91
Padlan, C.S., Malashkevich, V., Almo, S.C., Levy, M., Brenowitz, M. & Girvin, M.E. (2014) An RNA aptamer possessing a novel monovalent cation-mediated fold inhibits lysozyme catalysis by inhibiting the binding of long natural substrates, RNA, 20(4), 447-61
Chen, C., Mitra, S., Jonikas, M., Martin, J., Brenowitz, M., Laederach, A. Biophys J. (2013) Understanding the role of 3-dimensional topology in determining the folding intermediates of group 1 introns, Biophys J. 104(6), 1326-37
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Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Forchheimer Building, Room 311
Bronx, NY 10461