Associate, Department of Microbiology & Immunology
The overall goal of my research is directed towards understanding how intracellular pathogenic microorganisms metabolize in host tissues. The model for these studies is Mycobacterium tuberculosis. Important questions in my research focus on:
(i) Determining the substrates used by Mycobacterium tuberculosis during slow growth and non-replicating persistence in host tissues.
(ii) Elucidation of the metabolic and signaling pathways required for slow growth of M. tuberculosis and metabolism under hypoxia.
My research approach is strongly based on bacterial physiology and molecular microbiology. I am using a continuous culture system combined with transcriptomics and metabolomics to study mycobacterial metabolism.
Greening C, Berney M, Hards K, Cook GM, Conrad R. A soil actinobacterium scavenges atmospheric H2 using two membrane-associated, oxygen-dependent [NiFe] hydrogenases. PNAS Epub 2014 March 3, doi:10.1073/pnas/1320586111
Berney M, Cook GM. Respiration and Oxidative Phosphorylation in Mycobacteria.Advances in Photosynthesis and Respiration. In Advances in Photosynthesis and Respiration volume 29. Springer, Dordrecht, The Netherlands ISSN 1572-0233 IN PRESS (2014).
Lagautriere T, Bashiri G, Paterson NG, Berney M, Cook GM, Baker EN. Characterization of the proline utilization pathway in Mycobacterium tuberculosis through structural and functional studies. Acta Crystallographica Section D IN PRESS (2014).
Cook GM, Hards K, Vilcheze C, Hartman T, Berney M. Energetics of Respiration and Oxidative Phosphorylation in Mycobacteria. In Molecular Genetics of Mycobacteria, Hatfull, G. and Jacobs, Jr., W.R. (Eds) ASM Press, Washington DC. IN PRESS (2014).
Berney M, Greening C, Hards K, and Cook GM. Three [NiFe] Hydrogenases confer metabolic flexibility in the obligate aerobe Mycobacterium smegmatis. Environmental Microbiology Epub 2013 Nov 1, DOI: 10.1111/1462-2920.1232
Berney M, Weimar MR, Heikal A, Cook GM. Regulation of Proline Metabolism in Mycobacteria and Its Role in Carbon Metabolism under Hypoxia. Molecular Microbiology 2012 84(4): 664-681; Epub 2012 April 22
McKenzie JL, Robson JR., Berney M, Smith T, Ruthe A, Arcus VL and Cook GM. A VapBC Toxin-Antitoxin Module is a Post-Transcriptional Regulator of Metabolic Flux in Mycobacteria. Journal of Bacteriology 2012 194(9): 2189-2204; Epub 2012 Feb 24
Taniguchi N, Suzuki T, Berney M,Yoshida M, Cook GM. The Regulatory C-terminal Domain of Subunit e of FoF1 ATP Synthase is Dispensable for Growth and Survival of Escherichia coli. Journal of Bacteriology 2011 April;193(8): 2046-2052.
Hammes F, Berney M, Egli T. Cultivation-independent assessment of bacterial viabilityAdvances in Biochemical Engineering and Biotechnology, High Resolution Microbial Single Cell Analytics 2011 124:123-150.
Berney M and GM Cook. Unique Flexibility in Energy Metabolism Allows Mycobacteria to Combat Starvation and Hypoxia. PLoS One 2010 Jan;5: e8614
Hümpel A., Gebhard, S., Cook GM, Berney M. The SigF Regulon in Mycobacterium smegmatis Reveals Roles in Adaptation to Stationary Phase, Heat and Oxidative Stress. Journal of Bacteriology 2010 May;192(10):2491-502
McMillan DGG, Keis S, Berney M, Cook GM. Non-Fermentative Thermoalkaliphilic Growth is Restricted to Alkaline Environments. Appl Environ Microbiol 2009 Dec;75:7649-7654
Cook GM, Berney M, Gebhard S, Heinemann M, Cox RA, Danilchanka O, Niederweis M. Physiology of Mycobacteria. Advances in Microbial Physiology 2009;55:81-182, 318-9
Bosshard F., Berney M., Scheifele M., Weilenmann HU, Egli T. Solar disinfection (SODIS) and subsequent dark storage of S. typhimurium and S. flexneri monitored by flow cytometry. Microbiology-SGM 2009 Apr;155:1310-1317
Berney M, Vital M, Hülshoff I, Weilenmann HU, Egli T, Hammes F. Rapid, cultivation-independent assessment of microbial viability in drinking water. Water Res. 2008 Aug;42(14):4010-8. Epub 2008 Jul 22.
Hammes F, Berney M, Wang Y, Vital M, Köster O, Egli T. Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes. Water Res. 2008 Jan;42(1-2):269-77. Epub 2007 Jul 14.
Berney M, Hammes F, Bosshard F, Weilenmann HU, Egli T. Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry. Appl Environ Microbiol. 2007 May;73(10):3283-90. Epub 2007 Mar 23.
Berney M, Weilenmann HU, Egli T. Adaptation to UVA radiation of E. coli growing in continuous culture. J Photochem Photobiol B. 2007 Feb 1;86(2):149-59. Epub 2006 Oct 19.
Berney M, Weilenmann HU, Simonetti A, Egli T. Efficacy of solar disinfection of Escherichia coli, Shigella flexneri, Salmonella Typhimurium and Vibrio cholerae. J Appl Microbiol. 2006 Oct;101(4):828-36.
Berney M, Weilenmann HU, Egli T. Gene expression of Escherichia coli in continuous culture during adaptation to artificial sunlight. Environ Microbiol. 2006 Sep;8(9):1635-47.
Berney M, Weilenmann HU, Egli T. Flow-cytometric study of vital cellular functions in Escherichia coli during solar disinfection (SODIS). Microbiology-SGM. 2006 Jun;152(Pt 6):1719-29.
Berney M, Weilenmann HU, Ihssen J, Bassin C, Egli T. Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection. Appl Environ Microbiol. 2006 Apr;72(4):2586-93.
Looser V, Hammes F, Keller M, Berney M, Kovar K, Egli T. Flow-cytometric detection of changes in the physiological state of E. coli expressing a heterologous membrane protein during carbon-limited fedbatch cultivation. Biotechnol Bioeng. 2005 Oct 5;92(1):69-78.
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Albert Einstein College of Medicine
Michael F. Price Center
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Bronx, NY 10461