Faculty Profile

Dr. Daria Shcherbakova, Ph.D.

Daria Shcherbakova, Ph.D.

Associate, Department of Anatomy & Structural Biology

Professional Interests

My research focuses on engineering of natural photoreceptors into fluorescent proteins, biosensors, and optogenetic tools. I am specifically interested in design of genetically encoded optical tools allowing non-invasive detection and activation of biological processes with deeply penetrating near-infrared light. Our near-infrared spectrally distinct proteins iRFPs have been applied to imaging of various cell types, tissues and animal models in basic and translational research, including cancer and stem cell studies, neuroscience, and parasitology. These probes enable multiple in vivo imaging modalities, from simple planar epifluorescence to tomographic and photoacoustic technologies. We are currently developing the next generation of iRFPs that will allow non-invasive visualization of molecular processes.

Selected Publications

  1. Yao J., Kaberniuk A.A., Li L., Shcherbakova D.M., Zhang R., Wang L., Li G., Verkhusha V.V. and Wang L.V. Multi-scale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe. Nature Methods 2016, 13: 67-73.
  2. Shcherbakova D.M., Baloban M., Pletnev S., Malashkevich V.N., Xiao H., Dauter Z., and Verkhusha V.V. Molecular basis of spectral diversity in near-infrared phytochrome-based fluorescent proteins. Chem Biol. 2015, 22: 1540-1551.
  3. Shcherbakova D.M., Baloban M., and Verkhusha V.V. Near-infrared fluorescent proteins engineered from bacterial phytochromes. Curr. Opin. Chem. Biol. 2015, 27:52-63.
  4. Shcherbakova D.M., Shemetov A.A., Kaberniuk A.A., and Verkhusha V.V. Natural photoreceptors as a source of fluorescent proteins, biosensors and optogenetic tools. Annu. Rev. Biochem. 2015, 84: 519-550.
  5. Rice W.L., Shcherbakova D.M., Verkhusha V.V., and Kumar A.T. Whole-body tomographic fluorescence lifetime imaging using near infra-red fluorescent proteins. Cancer Research 2015, 75: 1236-1243.
  6. Shcherbakova D.M., Sengupta P., Lippincott-Schwartz J., and Verkhusha V.V. Photocontrollable fluorescent proteins for superresolution imaging. Annu. Rev. Biophys. 2014, 43: 303-329.
  7. Krumholz A., Shcherbakova D.M., Xia, J., Wang L.V. and Verkhusha V.V. Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent proteins. Sci. Reports 2014, 4: 3939. 
  8. Shcherbakova D.M. and Verkhusha V.V. Near-infrared fluorescent proteins for multicolor in vivo imaging. Nature Methods 2013, 10: 751-754.
  9. Shcherbakova, D.M., Subach, O.M., and Verkhusha, V.V. Red fluorescent proteins: Advanced imaging applications and future design. Angew. Chem. Int. Ed. 2012, 51: 10724-10738.
  10. Shcherbakova, D.M., Hink, M.A., Joosen, L., Gadella, T.W., and Verkhusha, V.V. An orange fluorescent protein with a large Stokes shift for single-excitation multicolor FCCS and FRET imaging. J. Am. Chem. Soc. 2012, 134: 7913-7923.

More Information About Dr. Daria Shcherbakova

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Vlad Verkhusha Lab

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Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
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Ullmann Building, Room 1217
Bronx, NY 10461

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Research Information