Faculty Profile

Dr. Carolina Eliscovich, Ph.D.

Carolina Eliscovich, Ph.D.

Research Assistant Professor, Department of Medicine (Hepatology)

Research Assistant Professor, Department of Anatomy & Structural Biology

Areas of Research: We study single mRNA molecules in single cells using high resolution imaging techniques. We develop microscopy technology using fluorescent-labeled probes to visualize and quantify mRNAs within the cell and tissue.

Professional Interests

Dr. Eliscovich focuses her research towards to the elucidation of RNA regulatory mechanisms facilitated by RNA-binding proteins during development and disease. She is interested in develop imaging methods to study gene expression regulation in living cells and tissue. She has developed a robust methodology, named ‘super registration’, using single-molecule RNA Fluorescence in situ hybridization in combination with immunofluorescence (smFISH-IF) to determine the physical interaction between individual mRNA molecules and their binding proteins in single cells using standard wide-field microscopy. This imaging method extends biochemical-based studies on RNA-protein interactions by providing spatial information about where in the cells these interactions are likely to occur. This is especially important in neurons, in which RNA regulatory mechanisms play an essential role in the regulation of localized gene expression. Super registration represents a solution to the historical problem of colocalization of two colors using optical microscopy. The secret to the success was devising a method to correct the intrinsic aberration of the commercial microscope objectives, each of which is unique. This allows her the use of fluorescence imaging to characterize the interaction of two molecules while in their native environment.

She is also interested in explore heterogeneity in gene expression in the liver as a tissue model. Virtually nothing is known about how an mRNA is regulated in primary cells or in their native context in the tissue. By characterizing molecular events as they occur in intact tissue, Dr. Eliscovich uses modern imaging technology to examine cause-and-effect relationships and quantitative kinetics to build predictive models of gene expression. Application of single-molecule and single-cell analyses to histopathology promises to yield new insights into the pathobiology of liver disease.


Selected Publications

Eliscovich C, Shenoy SM, Singer RH. Imaging mRNA and protein interactions within neurons. Proc Natl Acad Sci USA 2017; 114(10):E1875-E1884. PMID: 28223507 PMCID: PMC5347572

Eliscovich C, Singer RH.  RNP transport in cell biology: the long and winding road. Curr Opin Cell Biol 2017; 45:38-46. PMID: 28258033 PMCID: PMC5482755

Wu B, Eliscovich C, Yoon YJ, Singer RH. Translation dynamics of single mRNAs in live cells and neurons. Science 2016; 352(6292):1430-5. PMID: 27313041 PMCID: PMC4939616

Eliscovich C, Buxbaum AR, Katz ZB, Singer RH. mRNA on the move: the road to its biological destiny. J Biol Chem 2013; 288(28):20361-8. PMID: 23720759 PMCID: PMC3711302

Bava FA, Eliscovich C, Ferreira PG, Miñana B, Ben-Dov C, Guigó R, Valcárcel J, Méndez R. CPEB1 coordinates alternative 3'-UTR formation with translational regulation. Nature 2013; 495(7439):121-5. PMID: 23434754

Ortiz-Zapater E, Pineda D, Martínez-Bosch N, Fernández-Miranda G, Iglesias M, Alameda F, Moreno M, Eliscovich C, Eyras E, Real FX, Méndez R, Navarro P. Key contribution of CPEB4-mediated translational control to cancer progression. Nat Med 2011; 18(1):83-90. PMID: 22138752

Novoa I, Eliscovich C, Belloc E, Mendez R.  Oogenesis: The Universal Process. Verlhac M, Villeneuve A, editors. UK: Wiley-Blackwell, A John Wiley & Sons, Ltd.; 2010. Chapter 8, Oocyte-Specific Translational Control Mechanisms; p.199-226.

Eliscovich C, Peset I, Vernos I, Méndez R. Spindle-localized CPE-mediated translation controls meiotic chromosome segregation. Nat Cell Biol 2008; 10(7):858-65. PMID: 18536713


More information about Dr. Carolina Eliscovich

     Liver Research Center - Imaging and Cell Structure Core

     Marion Bessin Liver Research Center

     Department of Anatomy and Structural Biology


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Research Information