Instructor, Department of Orthopaedic Surgery
Research Interest: I have been working in the field of Neuroscience since 2005, and my research focuses on the investigation of glia-neuron interactions in regard to protection of neuronal function and survival in various neuronal disorders, including neuropathic and inflammatory pain, epilepsy and Alzheimer’s. My current research focuses on the role of satellite glia cells in driving and controlling aberrant neuronal activity in inflammatory- and neuropathic induced pain, and the identification of new targets for successful pain therapies. The awareness of the important role of glia for normal neuronal function has increased in the neuroscience field in recent years, but their mode of action in modulating neuronal activity by gliotransmitter and cytokine release is not completely understood. I have not only a great interest in dissecting these important mechanisms in in vitro cell biological and protein biochemistry experiments, but am also fascinated by analyzing my hypotheses in animal models of human diseases using in vivo behavioral experiments as they represent the complexity of life.
Collaborations: Dept of Orthopedics (arthritis, low back pain), Dept of Urology (pelvic pain) and rodent behavior (Behavior core) to extend translational pain research at Einstein.
Techniques: development of translational disease models; rodent behavioral tests; imaging; confocal microscopy; biostatistics; live cell/tissue assays (ATP assay, Dye uptake); molecular biology (PCR arrays, qPCR), protein biochemistry (ELISAs and immunostaining), and calcium imaging experiments
Hanstein, R., Negoro, H., Patel, N.K., Charollais, A., Meda, P., Spray, D.C., Suadicani, S.O., Scemes, E. (2013): Promises and pitfalls of conditional Pannexin1 knockout mice. Front Pharmacol. May 9;4:61. doi: 10.3389/fphar.2013.00061. PMID: 23675350 PMCID: PMC3648696
Stumm, C., Hiebel, C., Hanstein, R., Purrio, M., Nagel, H., Conrad, A., Lutz, B., Behl, C., Clement, A.B. (2013): Cannabinoid receptor 1 deficiency in a mouse model of Alzheimer's disease leads to enhanced cognitive impairment despite of a reduction in amyloid deposition. Neurobiol Aging. Nov;34(11):2574-84. doi: 10.1016/j.neurobiolaging.2013.05.027. Epub 2013 Jul 6. PMID: 23838176
Spray, D.C., Hanstein, R., Lopez-Quintero, S.V., Stout, R., Suadicani, S.O., Thi, M.M. (2012): Gap junctions and Bystander Effects: Good Samaritans and executioners. Wires Membrane Transport and Signaling, Jan;2(1):1-15. Epub 2012 Dec 11. PMID: 23565352 PMCID: PMC3614363
Rauschendorf, M.A., Zimmer, J., Hanstein, R., Dickemann, C., Vogt P.H. (2011): Complex transcriptional control of the AZFa gene DDX3Y in human testis. Int J Androl, Feb;34(1):84-96. PMID: 20374305 PMCID: PMC3039753
Hanstein, R., Zhao, J.B., Basak, R., Smith, D.N., Zuckerman, Y.Y., Hanani, M., Spray, D.C., Gulinello, M. (2010): Focal Inflammation Causes Carbenoxolone-Sensitive Tactile Hypersensitivity in Mice. Open Pain J. Jan;3:123-133. PMID: 21151805 PMCID: PMC2999631
Hanstein, R., Trotter, J., Behl, C., Clement, A.B. (2009): CRH Protects Neurons towards Oxidative Stress by Increasing Connexin43 Expression and Promoting Gap Junctional Coupling. Molecular Endocrinology, Sep;23(9):1479-93. PMID: 19460861 PMCID: PMC2737551
Hanstein, R., Lu, A., Wurst, W., Holsboer, F., Deussing, J., Clement, A.B., Behl, C. (2008): Transgenic overexpression of corticotropin releasing hormone provides partial protection against neurodegeneration in an in vivo model of acute excitotoxic stress. Neuroscience, Oct 15;156(3):712-21. PMID: 18708129
Vogt P.H., Lima-Falcao, C., Hanstein, R., Zimmer, J. (2008): The AZF proteins. Review Article. International Journal of Andrology; Aug;31(4):383-94. PMID: 18462373
Clement, A.B., Hanstein, R., Schröder, A., Nagel, H., Endres, K., Fahrenholz, F., Behl, C. (2008): Effects of neuron-specific ADAM10 modulation in an in vivo model of acute excitotoxic stress. Neuroscience; Mar 18;152(2):459-68. PMID: 18276079
Rajpert-De Meyts E., Hanstein, R., Jørgensen, N., Græm, N., Vogt, P.H., Skakkebæk, N.E. (2004): Differential developmental expression of the OCT-3/4 (POU5F1) in normal and dysgenetic human gonads. Human Reproduction; Jun 19(6):1338-44. PMID: 15105401
Wahezi, S.E., Hanstein, R., Spray, D.C. (2013): Understanding the pathways of pain. Chapter 12. In: Basic Science of Spinal Diseases. Eds Dr. Alok Sharan, Dr. Alexander Vaccaro and Dr. Simon Tang. Jaypee Brothers Medical Publishers Pvt. Ltd., 1 edition
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