Research Assistant Professor, Department of Pathology
Research conducted in the lab of Dr. Laura Santambrogio in the Pathology Department/AECOM.
1. Mass spectrometry of proteins and peptides from different biological fluids (lymph, plasma, synovial fluid), biomarkers discovery; label and label free quantitative proteomics aproaches for monitoring redox changes in the proteomes during aging and autoimmune diseases/cancer.
2. Enzyme based peptidomics coupled with nanoLC/MS/MS for discovery of neoepitopes derived from the processing of extracellular proteins (mainly collagen I and II (human)) by metalloproteases, cathepsins and other proteases.
3. PTM (posttranslational modification) scanning and Redox proteomics (PTM of oxidative stress) assays for profiling of peptides/proteins in dendritic and other immune cells in aging, autoimmune diseases and immuno-compromised pathological states.
4. Biophysical methods for study the proteins folding/unfolding under oxidative stress (oxygen free radical modified proteins): circular dichroism for secondary structural changes during oxidation of collagens, fluorescence studies of the tertiary structures changes during proteins unfolding under pathological conditions (such as reactive oxygen species generated during oxidative stress in aging).
Annexin A2 promotes phagophore assembly by enhancing Atg16L(+) vesicle biogenesis and homotypic fusion. Morozova K, Sidhar S, Zolla V, Clement CC, Scharf B, Verzani Z, Diaz A, Larocca JN, Hajjar KA, Cuervo AM, Santambrogio L. Nat Commun. 2015 Jan 19;6:5856
Hydrodynamic size-based separation and characterization of protein aggregates from total cell lysates. Tanase M, Zolla V, Clement CC, Borghi F, Urbanska AM, Rodriguez-Navarro JA, Roda B, Zattoni A, Reschiglian P, Cuervo AM, Santambrogio L. Nat Protoc. 2015 Jan;10(1):134-48.
Molecular analysis of chromium and cobalt-related toxicity. Scharf B, Clement CC, Zolla V, Perino G, Yan B, Elci SG, Purdue E, Goldring S, Macaluso F, Cobelli N, Vachet RW, Santambrogio L. Sci Rep. 2014 Jul 17;4:5729
Lymph Self-Antigen Repertoire. Clement CC, Santambrogio L. Front Immunol. 2013 Dec 16;4:424. eCollection 2013. Review.
Age-related carbonylation of fibrocartilage structural proteins drives tissue degenerative modification. Scharf B, Clement CC, Yodmuang S, Urbanska AM, Suadicani SO, Aphkhazava D, Thi MM, Perino G, Hardin JA, Cobelli N, Vunjak-Novakovic G, Santambrogio L. Chem Biol. 2013 Jul 25;20(7):922-34. doi: 10.1016/j.chembiol.2013.06.006.
Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics. Clement CC, Aphkhazava D, Nieves E, Callaway M, Olszewski W, Rotzschke O, Santambrogio L. J Proteomics. 2013 Jan 14;78:172-87. doi:10.1016/j.jprot.2012.11.013. Epub 2012 Nov 30.
Age-related oxidative stress compromises endosomal proteostasis. Cannizzo ES, Clement CC, Morozova K, Valdor R, Kaushik S, Almeida LN, Follo C, Sahu R, Cuervo AM, Macian F, Santambrogio L. Cell Rep. 2012 Jul 26;2(1):136-49. doi: 10.1016/j.celrep.2012.06.005. Epub 2012 Jul 12.
Annexin A2 binds to endosomes following organelle destabilization by particulate wear debris. Scharf B, Clement CC, Wu XX, Morozova K, Zanolini D, Follenzi A, Larocca JN, Levon K, Sutterwala FS, Rand J, Cobelli N, Purdue E, Hajjar KA, Santambrogio L. Nat Commun. 2012 Mar 27;3:755. doi: 10.1038/ncomms1754.
Oxidative stress, inflamm-aging and immunosenescence. Cannizzo ES, Clement CC, Sahu R, Follo C, Santambrogio L. J Proteomics. 2011 Oct 19;74(11):2313-23. doi: 10.1016/j.jprot.2011.06.005. Epub 2011 Jun 21. Review.
Microautophagy of cytosolic proteins by late endosomes. Sahu R, Kaushik S, Clement CC, Cannizzo ES, Scharf B, Follenzi A, Potolicchio I, Nieves E, Cuervo AM, Santambrogio L. Dev Cell. 2011 Jan 18;20(1):131-9. doi: 10.1016/j.devcel.2010.12.003. Erratum in: Dev Cell. 2011 Mar 15;20(3):405-
The lymph as a pool of self-antigens. Clement CC, Rotzschke O, Santambrogio L. Trends Immunol. 2011 Jan;32(1):6-11. doi: 10.1016/j.it.2010.10.004. Epub 2010 Dec 1.
An expanded self-antigen peptidome is carried by the human lymph as compared to the plasma. Clement CC, Cannizzo ES, Nastke MD, Sahu R, Olszewski W, Miller NE, Stern LJ, Santambrogio L. PLoS One. 2010 Mar 26;5(3):e9863. doi: 10.1371/journal.pone.0009863.
Endosomal damage and TLR2 mediated inflammasome activation by alkane particles in the generation of aseptic osteolysis. Maitra R, Clement CC, Scharf B, Crisi GM, Chitta S, Paget D, Purdue PE, Cobelli N, Santambrogio L. Mol Immunol. 2009 Dec;47(2-3):175-84. doi: 10.1016/j.molimm.2009.09.023. Epub 2009 Oct 4.
Immunogenecity of modified alkane polymers is mediated through TLR1/2 activation. Maitra R, Clement CC, Crisi GM, Cobelli N, Santambrogio L. PLoS One. 2008 Jun 18;3(6):e2438. doi: 10.1371/journal.pone.0002438.
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More Information About Dr. Cristina Clement
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Albert Einstein College of Medicine
Jack and Pearl Resnick Campus
1300 Morris Park Avenue
Forchheimer Building, Room 138
Bronx, NY 10461