The Molecular Pathology rotation is designed to provide the Pathology residents with the basic knowledge of the principles of molecular biology/genetics and applications of molecular techniques to clinical laboratory testing. It covers nucleic acid basics, isolation of nucleic acids, Southern blot analysis, PCR, reverse transcription, real-time PCR, fragment analysis, capillary and gel electrophoresis, melting curve, DNA sequencing, array CGH (comparative genomic hybridization), microarray, assay design, QA/QC issues, interpretation, troubleshooting, etc. The rotation provides hand-on experience. A short and publishable clinical research project is encouraged.
The Molecular Pathology Laboratory at Montefiore Medical Center offers a broad menu of molecular/genetic testing in the areas of hematopathology/oncology, microorganism detection, genetics, genomics, pharmacogenetics, and engraftment monitoring. It is a regional reference laboratory. Techniques used range from traditional DNA hybridization methods to state-of-the-art technologies, including DNA sequencing, quantitative PCR, DNA fragment analysis by capillary electrophoresis, array CGH and microarray. The laboratory is equipped with ABI DNA sequencer, ABI real-time PCR equipment, Roche Light Cycler, Agilent array scanner and Illumina bead array reader. The laboratory provides timely reporting of results and is staffed for full consultation in all areas of testing. The laboratory is also committed to developing molecular technologies that will ensure the continued quality of clinical testing in the rapidly progressing field of molecular pathology/genetics. In addition, the laboratory functions as a molecular research core facility.
The director, Dr. Qiulu Pan, Assistant Professor of Pathology, is certified in clinical molecular genetics and clinical cytogenetics by the American Board of Medical Genetics and molecular diagnosis by the American Board of Clinical Chemistry. He received his medical training, master’s degree and PhD training from Zhejiang University in China and worked as a pediatric oncologist/hematologist there. He also received his postdoctoral training in molecular biology from College of Physicians & Surgeons of Columbia University and St. Jude Children’s Research Hospital and finished his clinical fellowship training at Memorial Sloan-Kettering Cancer Center & Weill Medical College of Cornell University. He is the author of about 20 research articles.
Director: Qiulu Pan, MD, PhD
Montefiore Medical Center
Department of Pathology
111 East 210th Street
Bronx, NY 10467
Residents are invited to participate in a short and publishable clinical research project. We encourage residents to be involved with project design, bench work, data analysis and manuscript writing. Examples of the projects that recent residents/fellows have worked include:
- Dr. Alina Dulau: Rapid polymerase chain reaction based detection of all length polymorphisms in the UGT 1A1 gene promoter.
- Dr. Eric Gorman: Comparative evaluation of three assays for JAK2V617F mutation analysis.
- Dr. Allen Leung: Genetic analysis of CYP450 2C9 and VKORC1 for patients with Factor V Leiden and Prothrombin mutations.
- Dr. Keyur Patel: Comparison of BIOMED-2 versus home-brew PCR assays for T cell receptor gamma gene rearrangements.
- Dr. Naomi Yachelevich: Delineation of a recombinant chromosome 10, trisomic for 10p and monosomic for 10q using aCGH: A case report and review of the literature.
Real Time PCR