Spotlight

Dominick P. Purpura Department of Neuroscience

Cellular and Molecular Neuroimaging Core

Go to the Cellular and Molecular Neuroimaging Core Website 

     The Rose F. Kennedy Center Cellular and Molecular Neuroimaging Core has existed at Albert Einstein College of Medicine for over 20 years supported by the Department of Neuroscience, Center grants from the National Institute of Child Health and Development, and other external funds. The overall goal of the Core is to provide investigators a comprehensive package of expert guidance, training and assistance in cell and tissue preparative techniques, optical imaging, electron and related microscopy techniques, and image processing. Services include: specimen preparation; consultation on experimental design, data acquisition and analysis; training and assistance with major equipment and software use; and access to advanced media presentation technologies. The staff provides exceptionally personalized service and welcomes investigators from all areas of biology and medicine, in addition to providing scientific expertise for neuroscience studies.

     Consultation, training, and assistance are available for all services and equipment listed below. Please visit our website to schedule an appointment.

Director:
Kostantin Dobrenis, Ph.D
tel. 718-430-4027

Staff:
Kevin Fisher (Image Analysis)
tel. 718-430-2455
Ken Nguyen (Electron Microscopy)
tel. 718-430-4095

Associate Director:
David H. Hall, Ph.D
tel. 718-430-2195


Andrew Smith (Optical Microscopy and Laser Microdissection)
tel. 718-430-2046

 

Cell and Tissue Preparation:
 

    Equipment
  • Veritas 704 Laser Microdissection and Laser Capture Platform
  • AO 860 Sliding Freezing Microtome
  • Leica CM30505 Cryostat
  • Leica VT1000S Vibratome
  • RMC Powertome XL ultramicrotome
  • Sorvall MT2B ultramicrotome
  • Bal-Tec HM 100
  • RMC FS High Pressure Freezer and Freeze Substitution Device
  • Denton evaporator
  • Cressington Freeze Fracture Device
  • Pelco Biowave microwave
  • Pelco Cryobox


  • Capabilities
  • Laser assisted tissue region and single cell isolation for gene expression and proteomics studies
  • Large tissue sample sectioning
  • Thick, thin and ultrathin section preparation
  • Microwave irradiative fixation
  • High pressure freezing (HPF) and freeze fracture preparation
Other Major Resources:
    Equipment
  • Helios Biolistics “Gene Gun” system
  • Flexstation II auto-sample handler and fluorescence plate reader
  • Microscope incubation systems: Bioptecs closed perfusion system; PDMI-2 multi-injection culture chamber; Warner DH40i and TB-3 bipolar thermoregulated culture chambers
  • Picospritzer


  • Capabilities
  • Facile delivery of naked DNA to cells and tissues
  • Automated liquid handling for multiplate fluorescence assays
  • Multiwavelength quantitative fluorometry for soluble and cell fluorescence samples up to 384-well plates
  • Multiple microscope incubation systems for optimal live cell and tissue recording arrangements
  • Micropipette pressure injector for single cell perfusion and microinjection
Data Analysis/Visualization:
  • Amira (3D/4D visualization)
  • AutoAligner (plane realignment)
  • AutoQuant X (widefield deconvolution)
  • Imaris (3D/4D visualization/quantification)
  • Matlab (data analysis, FMRI)
  • Metamorph (quantitative image analysis, reconstruction and scripting)
Media Generation:
  • Epson 9890 wideformat printer
  • Adobe Creative Suite (Photoshop, Illustrator, Acrobat, Flash, Premiere)

 

Imaging: Widefield Microscopy:
 

    Equipment
  • Olympus AX70 upright microscope
  • Olympus Multi-Time/Sample (MTS) inverted microscope system
  • Olympus BX41 upright two-headed microscope


  • Capabilities
  • Autofocus
  • Fluorescence, brightfield, phase contrast and DIC
  • Automated multiwavelength and time-lapse image acquisition for multi-sample specimens including up to 384-well multiwells
  • Dual-viewing for teaching and consultation
Imaging: Stereology & Morphology:
    Equipment
  • MBF Bioscience Stereo Investigator workstation
  • MBF Bioscience Neurolucida workstation


  • Capabilities
  • Three-dimensional neuronal evaluation and reconstruction
  • Brightfield and epifluorescence stereologically sound cell counts
  • Volume estimations
Imaging: Confocal Microscopy:
    Equipment
  • Olympus Fluoview 500
  • Zeiss LSM 510 Meta Duo V2


  • Capabilities
  • Sequential laser scanning
  • 12 laser line availability
  • Ultrafast scanning (120f/s for 512 x 512 pixels, >30,000 lines/s)
  • Nanopositioning ultrafast Piezo objective Z drive
  • Spectral Imaging
  • Up to 5D acquisition (x,y,z,t,λ)
  • Simultaneous photomanipulation and image acquisition
  • Tiling functions for high resolution large field acquisition
  • Custom- and multi-region scanning
  • Programmable software for automated acquisition routines
  • Specialized FRET and FRAP software modules
Imaging: Electron Microscopy:
    Equipment
  • Philips CM10 Transmission Electron Microscope
  • FEI Technai 20 Transmission Electron Microscope
  • Focused Ion Beam Milling (FIB/SEM)


  • Capabilities
  • Electron tomography
  • Serial blockface imaging
  • Focused Ion Beam Milling (FIB/SEM)
  • Serial thin section analysis with Elegance software

 

 

Contact Us

Rose F. Kennedy Center

1410 Pelham Pkwy S.

Rm 903, Bronx, NY 10461

Phone: 718.430.3415

Fax: 718.430.8821

 
 
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