Professor, Department of Cell Biology
Chanin Bldg., Room 403
718.430.3527
matthew.scharff@einstein.yu.edu
Research Interest
Recent Publications
Biosketch
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1. Error-prone
Candidates Vie for Somatic Mutation. Poltoratsky, V., Goodman, M.E., and
Scharff, M.D., J. Exp. Med. 192: 27-30, 2000.
2.
Activation-induced cytidine deaminase turns on somatic hypermutation in
hybridomas. Alberto Martin, Philip D. Bardwell, Caroline J. Woo, Manxia Fan*,
Marc J. Shulman, Matthew D. Scharff. Nature 415: 802, 2002.
3. Induction of
somatic hypermutation is associated with modifications in immunoglobulin
variable region chromatin. Caroline J. Woo, Alberto Martin, and Matthew D.
Scharff Immunity 19:479-489, 2003. [html]
[pdf]
4.
Activation-induced cytidine deaminase deaminates deoxycytidine on
single-stranded DNA but requires the action of RNase. Ronda Bransteitter, Phuong
Pham, Matthew D. Scharff and Myron F. Goodman. Proc. Natl. Acad.
Sci. USA 100: 4102-4107, 2003
5. Altered
somatic hypermutation and reduced class switch recombination in Exonuclease
1-mutant mice. Philip D Bardwell, Caroline J Woo, Kaichun Wei, Ziqiang Li,
Alberto Martin, Stephen Z Sack, Tchaiko Parris, Winfried Edelmann, and
Matthew D Scharff Nat. Immunol. 5: 224-229, 2004
6. Differential regulation of histone acetylation and
generation of mutations in switch regions is associated with Ig class switching
Li, Z, Luo, Z and Scharff, M.D.Proc Natl Acad Sci U S A.101:15428-33.
2004. (PMCID: PMC524454). [PMC
html] [pdf]
7. Complex
regulation of somatic hypermutation by cis-acting sequences in the endogenous
IgH gene in hybridoma cells. D. Ronai, M.D. Iglesias-Ussel, M. Fan, M.J. Shulman,
M.D. Scharff. Proc Natl Acad Sci U S A. 102:11829-11834, 2005. (PMCID:
PMC1188009). [PMC
html] [pdf]
8. The mismatch
repair protein Msh6 plays a role in the targeting of AID to the Ig locus in
vivo. Z. Li, C. Zhao, Z. Polonskaya, M. Zhuang, G. Yang, M.D. Iglesias-Ussel,
Z. Luo, W. Edelmann, and M.D. Scharff. Immunity 24:393-403, 2006
9. Detection of
Chromatin-Associated ssDNA in Regions Targeted for Somatic Hypermutation. Ronai,
D., Iglesias-Ussel. M.D., Fan, M., Li, Z., Martin, A and M. D. Scharff.J.
Exp. Med. 204:181-190, 2007. (PMCID: PMC2118410). [PMC
html] [pdf]
10. The Biochemistry of Somatic Hypermutation. Peled, J.U.,
F. L. M. D. Kuang, Iglesias-Ussel, S. Roa, S.L. Kalis, M.F. Goodman and M.D.
Scharff. Annual Reviews of Immunology. 26:481-511, 2008. [html]
[pdf]
11. Ubiquitylated
PCNA plays a role in somatic hypermutation and class-switch recombination and is
required for meiotic progression. Roa S, Avdievich E, Peled JU, Maccarthy T,
Werling U, Kuang FL, Kan R, Zhao C, Bergman A, Cohen PE, Edelmann W, Scharff
MD. Proc Natl Acad Sci U S A. 105:16248-53. 2008. (PMCID: PMC2571010) Free PMC ArticleFree
text.
12. SHMTool: A
webserver for comparative analysis of somatic hypermutation datasets. Maccarthy
T, Roa S, Scharff MD, Bergman A. DNA Repair (Amst). 8:137-41, 2009. (PMCID:
PMC2659805)Free PMC ArticleFree
textThe web site with this algorithm is(http://scb.aecom.yu.edu/shmtool)
13. H3 trimethyl K9
and H3 acetyl K9 chromatin modifications are associated with class switch
recombination. Kuang FL, Luo Z, Scharff MD. Proc. Natl. Acad. Sci. USA
106:5288-93, 2008 (PMCID:
PMC2654022) Free PMC ArticleFree
text.
14. V-region
mutation in vitro, in vivo and in silico reveal the importance of
the enzymatic properties ofAID and the sequence environment. Thomas
MacCarthy, Susan Kalis, Sergio Roa, Phuong Pham, Myron F. Goodman, Matthew D.
Scharff, and Aviv Bergman. Proc Natl Acad Sci USA 106:8629-34, 2009 (PMCID:
PMC2682541).
Free PMC Article Free text
15.
Molecular characterization of hybridoma subclones spontaneously switching
at high frequencies in vitro. Iglesias-Ussel MD, Zavadil J, Scharff MD. J
Immunol Methods. 350:71-78, 2009. (PMCID: PMC2762006) Free PMC ArticleFree
text
16.
The
RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination.Ramachandran
S, Chahwan R, Nepal RM, Frieder D, Panier S, Roa S, Zaheen A, Durocher D,
Scharff MD, Martin A.Proc Natl Acad Sci U S A. 107:809-14. 2010
PMID: 20080757 Free PMC Article Free text
17.
Requirement for cyclin D3 in germinal center formation and function.
Peled, J.U., Yu, J.J., Venkatesh, J., Bi, E., Ding, B.B., Krupski-Downs, M., Shaknovich,
R., Sicinski, P., Diamond, B., Scharff, M.D., Ye, B.H. Cell Research 6:
631-46. 2010 PMID: 20404856 Free PMC ArticleFree
text
18.
MSH2/MSH6 complex
promotes error-free repair of AID-induced dU:G mispairs as well as error-prone
hypermutation of A:T sites. Roa S, Li Z, Peled JU, Zhao C,
Edelmann W, Scharff MD.PLoS One. 2010 Jun 17;5(6):e11182.PMID:
20567595 Free PMC ArticleFree
text
19.
PMS2 endonuclease
activity has distinct biological functions and is essential for genome
maintenance.van Oers JM, Roa S, Werling U, Liu Y, Genschel J, Hou
H Jr, Sellers RS, Modrich P, Scharff MD, Edelmann W.Proc Natl Acad Sci U S A.
107:13384-9, 2010PMID: 20624957 Free PMC ArticleFree
text
20.
Msh6 protects mature B
cells from lymphoma by preserving genomic stability. Peled JU,
Sellers RS, Iglesias-Ussel MD, Shin DM, Montagna C, Zhao C, Li Z, Edelmann W,
Morse HC 3rd, Scharff MD. Am J Pathol.177(5):2597-608, 2010. Epub 2010 Oct 7.
PMID: 20934970 [PubMed - in process] PMC:
2966815
21.
A requirement for FcγR
in antibody-mediated bacterial toxin neutralization.Abboud N,
Chow SK, Saylor C, Janda A, Ravetch JV, Scharff MD, Casadevall A.J Exp Med.
207(11):2395-405, 2010. PMID: 20921285PMC2964574
22.
Generation, characterization and
epitope mapping of neutralizing and protective monoclonal antibodies against
staphylococcal enterotoxin B induced lethal shock. Avanish K. Varshney, Xiaobo
Wang, Emily Cook, Kaushik Dutta, Matthew D. Scharff, Michael J. Goger, and
Bettina C. Fries. J. Biol. Chem. published January 13, 2011 [Epub ahead
of print]PMID: 21233204 [PubMed - as supplied by
publisher] Free ArticleFree
Text
23.
Recombinase-mediated
cassette exchange as a novel method to study somatic hypermutation in ramos
cells. Baughn LB, Kalis SL, MacCarthy T, Wei L, Fan M, Bergman A,
Scharff MD. MBio. 2011 Oct 11;2(5). pii: e00186-11. doi: 10.1128/mBio.00186-11.
Print 2011. PMID: 21990614 [PubMed - in process]
Free Article
24.
Intra-clonal
complexity in chronic lymphocytic leukemia: fractions enriched in recently
born/divided and older/quiescent cells.Calissano C, Damle RN,
Marsilio S, Yan XJ, Yancopoulos S, Hayes G, Emson C, Murphy EJ, Hellerstein MK,
Sison C, Kaufman MS, Kolitz JE, Allen SL, Rai KR, Ivanovic I, Dozmorov IM, Roa
S, Scharff MD, Li W, Chiorazzi N.Mol Med. 2011 Sep 23. doi:
10.2119/molmed.2011.00360. [Epub ahead of print]PMID: 21968788 [PubMed - as
supplied by publisher]
Free Article
25.
Mismatch-mediated
error prone repair at the immunoglobulin genes. Chahwan R,
Edelmann W, Scharff MD, Roa S.Biomed Pharmacother. 2011 Dec; 65(8):529-36. ]http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235044/
26.
The use of Bcl-2
over-expression to stabilize hybridomas specific to the HERG potassium channel.
Sroubek J, Krishnan Y, Chinai J, Buhl S, Scharff MD, McDonald TV. J Immunol
Methods. 2011 Nov 15. [Epub ahead of print]PMID: 22107967 [PubMed - as supplied
by publisher
27.
The ATPase activity of
MLH1 is required to orchestrate DNA double-strand breaks and end processing
during class switch recombination. Chahwan R, van Oers JM, Avdievich
E, Zhao C, Edelmann W, Scharff MD, Roa S. J Exp Med. 209:671-8, 2012
28.
Germline deletion of
Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific
regulation, rearrangement, and insulation of the Igh locus.Volpi
SA, Verma-Gaur J, Hassan R, Ju Z, Roa S, Chatterjee S, Werling U, Hou H Jr, Will
B, Steidl U, Scharff M, Edelman W, Feeney AJ, Birshtein BK. J Immunol.
188(6):2556-66, 2012 PMC3430471
29.
AIDing antibody diversity
by error-prone mismatch repair. Chahwan R, Edelmann W, Scharff MD,
Roa S.Semin Immunol. 2012 Aug;24(4):293-300. Epub 2012 Jun 14.PMID:
22703640 PMC3422444
30. IGHV unmutated and
mutated chronic lymphocytic leukemia cells produce activation-induced deaminase
protein with a full range of biologic functions. Patten PE, Chu CC,
Albesiano E, Damle RN, Yan XJ, Kim D, Zhang L, Magli AR, Barrientos J, Kolitz JE,
Allen SL, Rai KR, Roa S, Mongini PK, Maccarthy T, Scharff MD, Chiorazzi
N. Blood. 2012 Oct 15. [Epub ahead of print] PMID:
23071276 PMC3520620
31. AID and Apobec3G
haphazard deamination and mutational diversity.Jaszczur M, Bertram JG,
Pham P, Scharff MD, Goodman MF.Cell Mol Life Sci. 2012 Nov 22. [Epub
ahead of print]PMID: 23178850 [PubMed - as supplied
by publisher]
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