Department of Cell Biology

Matthew D. Scharff, M.D. -- Recent Publications


Professor, Department of Cell Biology
Chanin Bldg., Room 403

Research Interest 
Recent Publications 



1.    Error-prone Candidates Vie for Somatic Mutation. Poltoratsky, V., Goodman, M.E., and Scharff, M.D., J. Exp. Med. 192: 27-30, 2000.

2.    Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas. Alberto Martin, Philip D. Bardwell, Caroline J. Woo, Manxia Fan*, Marc J. Shulman, Matthew D. Scharff.  Nature 415: 802, 2002.

3.    Induction of somatic hypermutation is associated with modifications in immunoglobulin variable region chromatin. Caroline J. Woo, Alberto Martin, and Matthew D. Scharff Immunity 19:479-489, 2003. [html] [pdf]

4.    Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase. Ronda Bransteitter, Phuong Pham, Matthew D. Scharff and Myron F. Goodman. Proc. Natl. Acad.         Sci. USA 100: 4102-4107, 2003

5.    Altered somatic hypermutation and reduced class switch recombination in Exonuclease 1-mutant mice.  Philip D Bardwell, Caroline J Woo, Kaichun Wei, Ziqiang Li, Alberto Martin, Stephen Z Sack, Tchaiko Parris, Winfried Edelmann, and Matthew D Scharff Nat. Immunol. 5: 224-229, 2004

6.    Differential regulation of histone acetylation and generation of mutations in switch regions is associated with Ig class switching Li, Z, Luo, Z and Scharff, M.D.Proc Natl Acad Sci U S A.101:15428-33. 2004. (PMCID: PMC524454). [PMC html] [pdf]

7.    Complex regulation of somatic hypermutation by cis-acting sequences in the endogenous IgH gene in hybridoma cells. D. Ronai, M.D. Iglesias-Ussel, M. Fan, M.J. Shulman, M.D. Scharff. Proc Natl Acad Sci U S A. 102:11829-11834, 2005. (PMCID: PMC1188009). [PMC html] [pdf]

8.    The mismatch repair protein Msh6 plays a role in the targeting of AID to the Ig locus in vivo. Z. Li, C. Zhao, Z. Polonskaya, M. Zhuang, G. Yang, M.D. Iglesias-Ussel, Z. Luo, W. Edelmann, and M.D. Scharff. Immunity 24:393-403, 2006

9.    Detection of Chromatin-Associated ssDNA in Regions Targeted for Somatic Hypermutation. Ronai, D., Iglesias-Ussel. M.D., Fan, M., Li, Z., Martin, A and M. D. Scharff.J. Exp. Med. 204:181-190, 2007. (PMCID: PMC2118410). [PMC html] [pdf]

10.  The Biochemistry of Somatic Hypermutation. Peled, J.U., F. L. M. D. Kuang, Iglesias-Ussel, S. Roa, S.L. Kalis, M.F. Goodman and M.D. Scharff. Annual Reviews of Immunology. 26:481-511, 2008. [html] [pdf]

11.  Ubiquitylated PCNA plays a role in somatic hypermutation and class-switch recombination and is required for meiotic progression. Roa S, Avdievich E, Peled JU, Maccarthy T, Werling U, Kuang FL, Kan R, Zhao C, Bergman A, Cohen PE, Edelmann W, Scharff MD. Proc Natl Acad Sci U S A. 105:16248-53. 2008. (PMCID: PMC2571010) Free PMC ArticleFree text.

12.  SHMTool: A webserver for comparative analysis of somatic hypermutation datasets.  Maccarthy T, Roa S, Scharff MD, Bergman A. DNA Repair (Amst). 8:137-41, 2009. (PMCID: PMC2659805)Free PMC ArticleFree textThe web site with this algorithm is(

13.  H3 trimethyl K9 and H3 acetyl K9 chromatin modifications are associated with class switch recombination.  Kuang FL, Luo Z, Scharff MD. Proc. Natl. Acad. Sci. USA 106:5288-93, 2008 (PMCID: PMC2654022) Free PMC ArticleFree text.

14.  V-region mutation in vitro, in vivo and in silico reveal the importance of the enzymatic properties ofAID and the sequence environment. Thomas MacCarthy, Susan Kalis, Sergio Roa, Phuong Pham, Myron F. Goodman, Matthew D. Scharff, and Aviv Bergman. Proc Natl Acad Sci USA 106:8629-34, 2009 (PMCID: PMC2682541). Free PMC Article Free text 

15.  Molecular characterization of hybridoma subclones spontaneously switching at high frequencies in vitro. Iglesias-Ussel MD, Zavadil J, Scharff MD. J Immunol Methods. 350:71-78, 2009. (PMCID: PMC2762006) Free PMC ArticleFree text 

16.  The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination.Ramachandran S, Chahwan R, Nepal RM, Frieder D, Panier S, Roa S, Zaheen A, Durocher D, Scharff MD, Martin A.Proc Natl Acad Sci U S A. 107:809-14. 2010 PMID: 20080757 Free PMC Article Free text 

17.  Requirement for cyclin D3 in germinal center formation and function. Peled, J.U., Yu, J.J., Venkatesh, J., Bi, E., Ding, B.B., Krupski-Downs, M., Shaknovich, R., Sicinski, P., Diamond, B., Scharff, M.D., Ye, B.H. Cell Research 6: 631-46. 2010 PMID: 20404856 Free PMC ArticleFree text 

18.  MSH2/MSH6 complex promotes error-free repair of AID-induced dU:G mispairs as well as error-prone hypermutation of A:T sites. Roa S, Li Z, Peled JU, Zhao C, Edelmann W, Scharff MD.PLoS One. 2010 Jun 17;5(6):e11182.PMID: 20567595 Free PMC ArticleFree text 

19.  PMS2 endonuclease activity has distinct biological functions and is essential for genome maintenance.van Oers JM, Roa S, Werling U, Liu Y, Genschel J, Hou H Jr, Sellers RS, Modrich P, Scharff MD, Edelmann W.Proc Natl Acad Sci U S A. 107:13384-9, 2010PMID: 20624957 Free PMC ArticleFree text 

20.  Msh6 protects mature B cells from lymphoma by preserving genomic stability. Peled JU, Sellers RS, Iglesias-Ussel MD, Shin DM, Montagna C, Zhao C, Li Z, Edelmann W, Morse HC 3rd, Scharff MD. Am J Pathol.177(5):2597-608, 2010. Epub 2010 Oct 7. PMID: 20934970 [PubMed - in process] PMC: 2966815 

21.  A requirement for FcγR in antibody-mediated bacterial toxin neutralization.Abboud N, Chow SK, Saylor C, Janda A, Ravetch JV, Scharff MD, Casadevall A.J Exp Med. 207(11):2395-405, 2010. PMID: 20921285PMC2964574 

22.  Generation, characterization and epitope mapping of neutralizing and protective monoclonal antibodies against staphylococcal enterotoxin B induced lethal shock. Avanish K. Varshney, Xiaobo Wang, Emily Cook, Kaushik Dutta, Matthew D. Scharff, Michael J. Goger, and Bettina C. Fries. J. Biol. Chem. published January 13, 2011 [Epub ahead of print]PMID: 21233204 [PubMed - as supplied by publisher] Free ArticleFree Text 

23.  Recombinase-mediated cassette exchange as a novel method to study somatic hypermutation in ramos cells. Baughn LB, Kalis SL, MacCarthy T, Wei L, Fan M, Bergman A, Scharff MD. MBio. 2011 Oct 11;2(5). pii: e00186-11. doi: 10.1128/mBio.00186-11. Print 2011. PMID: 21990614 [PubMed - in process] Free Article 

24.  Intra-clonal complexity in chronic lymphocytic leukemia: fractions enriched in recently born/divided and older/quiescent cells.Calissano C, Damle RN, Marsilio S, Yan XJ, Yancopoulos S, Hayes G, Emson C, Murphy EJ, Hellerstein MK, Sison C, Kaufman MS, Kolitz JE, Allen SL, Rai KR, Ivanovic I, Dozmorov IM, Roa S, Scharff MD, Li W, Chiorazzi N.Mol Med. 2011 Sep 23. doi: 10.2119/molmed.2011.00360. [Epub ahead of print]PMID: 21968788 [PubMed - as supplied by publisher] Free Article 

25.  Mismatch-mediated error prone repair at the immunoglobulin genes. Chahwan R, Edelmann W, Scharff MD, Roa S.Biomed Pharmacother. 2011 Dec; 65(8):529-36. ] 

26.  The use of Bcl-2 over-expression to stabilize hybridomas specific to the HERG potassium channel. Sroubek J, Krishnan Y, Chinai J, Buhl S, Scharff MD, McDonald TV. J Immunol Methods. 2011 Nov 15. [Epub ahead of print]PMID: 22107967 [PubMed - as supplied by publisher

27.  The ATPase activity of MLH1 is required to orchestrate DNA double-strand breaks and end processing during class switch recombination. Chahwan R, van Oers JM, Avdievich E, Zhao C, Edelmann W, Scharff MD, Roa S. J Exp Med. 209:671-8, 2012

28.  Germline deletion of Igh 3' regulatory region elements hs 5, 6, 7 (hs5-7) affects B cell-specific regulation, rearrangement, and insulation of the Igh locus.Volpi SA, Verma-Gaur J, Hassan R, Ju Z, Roa S, Chatterjee S, Werling U, Hou H Jr, Will B, Steidl U, Scharff M, Edelman W, Feeney AJ, Birshtein BK. J Immunol. 188(6):2556-66, 2012 PMC3430471 

29.  AIDing antibody diversity by error-prone mismatch repair. Chahwan R, Edelmann W, Scharff MD, Roa S.Semin Immunol. 2012 Aug;24(4):293-300. Epub 2012 Jun 14.PMID: 22703640 PMC3422444 

30.  IGHV unmutated and mutated chronic lymphocytic leukemia cells produce activation-induced deaminase protein with a full range of biologic functions. Patten PE, Chu CC, Albesiano E, Damle RN, Yan XJ, Kim D, Zhang L, Magli AR, Barrientos J, Kolitz JE, Allen SL, Rai KR, Roa S, Mongini PK, Maccarthy T, Scharff MD, Chiorazzi N. Blood. 2012 Oct 15. [Epub ahead of print] PMID: 23071276 PMC3520620 

31.  AID and Apobec3G haphazard deamination and mutational diversity.Jaszczur M, Bertram JG, Pham P, Scharff MD, Goodman MF.Cell Mol Life Sci. 2012 Nov 22. [Epub ahead of print]PMID: 23178850 [PubMed - as supplied by publisher] 



Click here to log in