Director: Jayanta Roy-Chowdhury, M.B., B.S.
Core Manager: David S. Neufeld, Ph.D.
Ullmann Building, Room 610
- The Cell Culture and Genetic Engineering Core has two components:
- The Cell Culture component provides Liver Research Center Investigators with the following:
- isolated liver cells
- well-characterized frozen stocks of cell lines
- specialized culture media, hormones and supplements, and matrix components for optimal cell growth
- equipment and instructions for use in isolation and culturing of various cell types derived from the liver
- The Genetic Engineering component assists Liver Center Investigators in developing recombinant viral vectors, (based on oncoretroviruses, lentiviruses, adenoviruses and baculoviruses) for use in their specific research, and maintains equipment for microscopy and assist in routine evaluation of cultured cells following genetic or other manipulations.
- Isolation and culture of primary hepatocytes from various animal species and humans
- Recombinant viral vectors for gene transfer (retroviral, lentiviral, baculoviral and adenoviral).
- Mycoplasma testing and distribution of well-characterized, mycoplasma-free frozen stocks of cell lines.
- Provision of components of specialized cell culture media, hormonal supplements and matrices.
- Maintenance of cell-isolation and cell-culture related equipment and instruction on their use.
- Provision of human embryonic stem cells.
- Maintenance of the digital recording Nikon microscope and assistance in its use.
- Culturing and provision of human embryonic stem cells for differentiation into liver cells.
- Provision of primary human liver cells isolated from resected liver segments and procured livers that are not used for transplantation.
- Assistance in genetically marking liver cells for identification after transplantation into animal models.
- Provision of primary human hepatocytes and endothelial cells for use in cellular, molecular, physiologic, pharmacologic and cell transplantation studies.