Diabetes Research Center

Hormone Assay Core


Location: Forchheimer 505

Director: Norman Fleischer, MD

Operations Manager: Manju Surana, PhD

Request Services: drtcria@einstein.yu.edu 

The Einstein Hormone Assay Core establishes an integrative framework to understand disorders in glucose and lipid metabolism.

Objectives

  1. Uniform, standardized, accurate, and cost-effective assays of various plasma hormones, adipokines, cytokines and chemokines in rodent models and human studies related to the regulation of energy balance, insulin sensitivity, carbohydrate and fatty acid metabolism
  2. Assessment of insulin secretion from a series of DRC-maintained mouse beta cell lines for local use and distribution to the scientific community at large
  3. A data storage and access system to provide results to investigators and monitor Core use. The data obtained directly relate to and integrate with the detailed metabolic analyses performed in the Animal Physiology and the Metabolomic and Stable Isotope Cores.
  4. Expert advice for DRC investigators on Core use, experimental design, education in assay performance, and data interpretation.
  5. Training

Methods

  1. RIA technology
  2. ELISA methodology (for a number of assays, especially those that require smaller plasma volumes or for analytes where RIA is not available or as reliable, such as cytokines)
  3. Luminex MultiAnalyte Profiling Technology (for multiplexing assays), a method that uses a mixture of distinct sets of uniquely colored fluorescent microspheres, which are identified by distinct fluorescent dyes by the Luminex dual capture flow analyzer. The different microsphere bead sets act as solid support for the immunoabsorbent assays with a green-fluorescent reporter dye.

Services

The Core carefully evaluates each assay before allowing investigators to request them. The Core also characterizes secretory physiology of -cell lines that are supplied to numerous laboratories throughout the scientific community so that investigators can correlate secretory physiology with biochemical and molecular studies. Investigators desiring to use the Core, who are not experienced in the capabilities of the methodology are encouraged to meet with the Core Director to discuss their needs and goals.

Current services provided, and related technologies, are as follows. Technologies are specified depending on investigator needs and in consultation with the Core director.

  • Insulin (RIA, ELISA and Luminex)
  • C-Peptide, (RIA, available for human and mouse)
  • Glucagon (RIA and Luminex)
  • Adiponectin (ELISA, RIA and Luminex). This assay was developed by Dr. Scherer in collaboration with Linco.
  • Human and Rodent Leptin (RIA)
  • Epinephrine and Norepinephrine (ELISA). This assay is measured by a recently developed ELISA which replaced the radioenzymatic assay that was utilized in the past.
  • Human Growth Hormone (ELISA)
  • Plasma Cortisol/Corticosterone (ELISA)
  • Resistin (RIA or Luminex, available for human, rat or mouse)
  • Cytokines, e.g., MCP-1, TNF-a, IL6, PAI-1, and CRP (ELISA or Luminex)
  • IGF-1 (RIA or ELISA)
  • Other Determinations by Luminex, e.g., cytokines-various custom and standard sets of assays as requested by investigators, provided there are satisfactory reagents available such as commercially available kits.
  • #946;TC Cell Physiology. The HAC tests the secretory physiology of a series of beta cell lines created at Einstein by Dr. Shimon Efrat and maintained here (e.g., TC-3 and TC tet).

Fees

Charges for assays provided by the HAC

ASSAY

TYPE

COST ($)

DRTC

Others

Insulin

RIA

6.00

9.00

Insulin

ELISA

10.00

15.00

Proinsulin

RIA

6.00

9.00

Human C-Peptide

RIA

6.00

9.00

Rat C-Peptide

RIA

6.00

9.00

Glucagon

RIA

6.00

9.00

Human/Mouse

Adiponectin

RIA

10.00

15.00

Pancreatic Polypeptide

RIA

15.00

22.50

Human Ghrelin

RIA

10.00

15.00

Cortisol

ELISA

8.00

12.00

IGF-1

ELISA

17.00

25.50

Corticosterone

ELISA

12.00

8.00

Human Growth Hormone

ELISA

10.00

15.00

Rat/Mouse Leptin

ELISA

14.00

21.00

Human Leptin

RIA

8.00

12.00

Human Resistin

ELISA

10.00

15.00

Mouse Resistin

ELISA

14.00

21.00

GLP-1 (total)

RIA

9.00

13.50

Epi/norepi

ELISA/RIA

37.00

55.50

TNFalpha (rat)

ELISA

9.00

13.50

IL-6 (rat)

ELISA

9.00

13.50

PAI-1

ELISA

18.00

27.00

Progesterone

RIA

7.00

10.50

Prioritization

Use of this Core is limited to investigators engaged in diabetes-related research at Albert Einstein College of Medicine and its affiliated Institutions. In addition, the Core will consider requests from investigators at other institutions where such services are not readily available. The Core generally supports projects funded by peer-reviewed grants, generally from the NIH, ADA, or JDRF.

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