The overall goal of the Einstein-Montefiore CFAR Flow Cytometry Core is to provide investigators with ready access to a wide array of advanced instruments for analytical flow cytometry and cell sorting in appropriate biosafety containment conditions for the analysis and isolation of specific cell populations for both clinical and basic research. 

 

Core Mission 

• To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards.
• To provide education, training and consultative services to assist CFAR investigators in the implementation of new and established flow cytometry techniques. 

Core Facilities 

• Analytical Flow Cytometers: FACScan DxP5, FACSCalibur DxP10, Standard FACSCalibur, FACSCanto II, BD LSR II (2), Partec CyCube
• High Speed Cell Sorters: MoFlo and FACSAria II
• BSL2-enhanced biocontained High Cell Sorter- FACSAria II
• iCys Laser Scanning Cytometer
• Nikon Optiphot Epifluorescence Microscope

Core Services 

• High-speed cell sorting of infected cells or tissues using FACSAria under biocontainment conditions
• Multiparameter flow cytometric analysis using MoFlo-XDP, FACSAria, FACSCanto II and FACSCalibur
• Laser scanning cytometry using Compucyte iCys
• Consultation on biocontainment requirements for cell sorting experiments
• Assistance with biohazard flow cytometry experimental design and data analysis 

Core Training Programs 

• Regularly provide practical courses and hands-on training in the use of all analytical flow cytometers.
• Provide training in operation of high speed cell sorters for advanced and suitably qualified facility users under biocontainment conditions.
• Provide consultation on design of experiments involving flow cytometry, and resources for processing and analyzing data.
• Organize and sponsor seminars and workshops by internal and invited external experts on various aspects of flow cytometry and related topics.

Current Core Charge Back Fees 

Instrument or Service	Standard Rate(per/hr)	CFAR Investigator Rate(per/hr)
FACScan or FACSCalibur, unassisted	$33.00	$26.40
FACScan or FACSCalibur, assisted	$72.00	$57.60
FACSCanto II, unassisted	$44.00	$35.20
FACSCanto II, assisted	$86.00	$68.80
LSR II, unassisted	$58.00	$46.40
LSR II, assisted	$86.00	$68.80
Cell Sorting (MoFlo, FACSAria)	$80.00	$64.00
iCys Laser Scanning Cytometer, data acquisition	$14.00	$11.20
MultiSet human lymphocyte subset analysis  (per sample analyzed)	$36.25	$29.00

 

•  Scheduling is available to registered AECOM Flow Cytometry Core Facility members only. Speak with the facility staff in order to obtain a login and pass code necessary to create reservations. To create a reservation to use any of the equipment or services in the facility, go to: http://lightbulb.aecom.yu.edu/phpFACS 
• To access Core services please contact Dr. Steven Porcelli.

Recent Core Publications

Eugenin EA, Clements JE, Zink MC, Berman JW.  Human immunodeficiency virus infection of human astrocytes disrupts blood-brain barrier integrity by a gap junction-dependent mechanism. J Neurosci. 2011;31:9456-65. PMCID: 3132881

Keller MJ, Madan RP, Torres NM, Fazzari MJ, Cho S, Kalyoussef S, Shust G, Mesquita PM, Louissaint N, Chen J, Cohen HW, Diament EC, Lee AC, Soto-Torres L, Hendrix CW, Herold BC.  A randomized trial to assess anti-HIV activity in female genital tract secretions and soluble mucosal immunity following application of 1% tenofovir gel. PLoS One 2011;6:e16475 PMCID:302683710. 

Berg KM, Litwin AH, Li X, Heo M, Arnsten JH. Lack of sustained improvement in adherence or viral load following a directly observed antiretroviral therapy intervention. Clinical Infectious Diseases 2011 Nov;53(9):936-43. PMCID: 3189166

Arnsten JH, Litwin AH, Berg KM.  Effect of directly observed therapy for highly active antiretroviral therapy on virologic, immunologic, and adherence outcomes: a meta-analysis and systematic review. J Acquir Immune Defic Syndr. 2011;56:e33-4.  PMID: 21150506

Herold BC, Mesquita PM, Madan RP, Keller MJ. Female genital tract secretions and semen impact the development of microbicides for the prevention of HIV and other sexually transmitted infections. Am J Reprod Immunol. 2011;65:325-33. PMCID: 3058365.