Center for AIDS Research

Flow Cytometry Core

CFAR Logo
porcelliThe overall goal of the Einstein-Montefiore CFAR Flow Cytometry Core is to provide investigators with ready access to a wide array of advanced instruments for analytical flow cytometry and cell sorting in appropriate biosafety containment conditions for the analysis and isolation of specific cell populations for both clinical and basic research particularly those infected with HIV-1. 

 

Core Mission 

  • To maintain the necessary research infrastructure needed for flow cytometry studies, with appropriate biosafety containment safeguards.
  • To provide education, training and consultative services to assist CFAR investigators in the implementation of new and established flow cytometry techniques. 

Core Facilities 

  • Analytical Flow Cytometers for multiparametric analysis of cellular populations and nanoparticles including FACScan DxP5, FACSCalibur DxP10, Standard FACSCalibur, FACSCanto II, BD LSR II (2), Partec CyCube
  • High Speed Cell Sorters for isolation of celluar populations including MoFlo and FACSAria II
  • BSL2-enhanced biocontained High Cell Sorter for the isolation of HIV-1-infected cells using a FACSAria II housed in a Baker Bio Protect III biosafety cabinet.
  • iCys Laser Scanning Cytometer for coupling high resolution images of singe cells with quantitative fluorescence measurements.
  • Nikon Optiphot Epifluorescence Microscope for visual imaging of fluorescently-labeled cells.

Core Services 

  • High-speed cell sorting of HIV-1-infected cells or tissues infected with other pathogens using FACSAria under BSL-2-enhanced biocontainment conditions
  • Multiparameter flow cytometric analysis using MoFlo-XDP, FACSAria, FACSCanto II and FACSCalibur
  • Laser scanning cytometry of tissue sections using Compucyte iCys Laser ScanningCytometer
  • Consultation on biocontainment requirements for cell sorting experiments
  • Assistance with biohazard flow cytometry experimental design and data analysis 

Core Training Programs 

  • Regularly provide practical courses and hands-on training to CFAR investigators in the use of all analytical flow cytometers.
  • Provide training in the operation of high speed cell sorters for advanced and suitably qualified facility users under biocontainment conditions.
  • Provide consultation on design of experiments involving flow cytometry, and resources for processing and analyzing data.
  • Organize and sponsor seminars and workshops by internal and invited external experts on various aspects of flow cytometry and related topics.

Current Core Charge Back Fees 

Instrument or Service 

Standard Rate(per/hr) 

CFAR Investigator Rate(per/hr) 

FACScan or FACSCalibur, unassisted 

$33.00 

$26.40 

FACScan or FACSCalibur, assisted 

$72.00 

$57.60 

FACSCanto II, unassisted 

$44.00 

$35.20 

FACSCanto II, assisted 

$86.00 

$68.80 

LSR II, unassisted 

$58.00 

$46.40 

LSR II, assisted 

$86.00 

$68.80 

Cell Sorting (MoFlo, FACSAria) 

$80.00 

$64.00 

iCys Laser Scanning Cytometer, data acquisition 

$14.00 

$11.20 

MultiSet human lymphocyte subset analysis  (per sample analyzed) 

$36.25 

$29.00 


Core Access

  •  Scheduling is available to registered CFAR Flow Cytometry Core Facility members only. Speak with the facility staff in order to obtain a login and pass code necessary to create reservations. To create a reservation to use any of the equipment or services in the facility, go to: http://lightbulb.aecom.yu.edu/phpFACS 
  • To access Core services please contact Dr. Steven Porcelli.

Recent Core Publications

1. Cordero RJ, Pontes B, Frases S, Nakouzi AS, Nimrichter L, Rodrigues ML, Viana NB, Casadevall A. Antibody binding to Cryptococcus neoformans impairs budding by altering capsular mechanical properties. Journal of immunology. 2013;190(1):317-23. Epub 2012/12/13. doi: 10.4049/jimmunol.1202324. PubMed PMID: 23233725; PubMed Central PMCID: PMC3529781.

2. Ly D, Kasmar AG, Cheng TY, de Jong A, Huang S, Roy S, Bhatt A, van Summeren RP, Altman JD, Jacobs WR, Jr., Adams EJ, Minnaard AJ, Porcelli SA, Moody DB. CD1c tetramers detect ex vivo T cell responses to processed phosphomycoketide antigens. J Exp Med. 2013;210(4):729-41. Epub 2013/03/27. doi: 10.1084/jem.20120624. PubMed PMID: 23530121; PubMed Central PMCID: PMC3620358.

3. Nixon B, Fakioglu E, Stefanidou M, Wang Y, Dutta M, Goldstein H, Herold BC. Genital Herpes Simplex Virus Type 2 Infection in Humanized HIV-Transgenic Mice Triggers HIV Shedding and Is Associated With Greater Neurological Disease. J Infect Dis. 2013. Epub 2013/08/31. doi: 10.1093/infdis/jit472. PubMed PMID: 23990571.

4. Nixon B, Stefanidou M, Mesquita PM, Fakioglu E, Segarra T, Rohan L, Halford W, Palmer KE, Herold BC. Griffithsin protects mice from genital herpes by preventing cell-to-cell spread. J Virol. 2013;87(11):6257-69. Epub 2013/03/29. doi: 10.1128/JVI.00012-13. PubMed PMID: 23536670; PubMed Central PMCID: PMC3648100.

5. Seay K, Qi X, Zheng JH, Zhang C, Chen K, Dutta M, Deneroff K, Ochsenbauer C, Kappes JC, Littman DR, Goldstein H. Mice Transgenic for CD4-Specific Human CD4, CCR5 and Cyclin T1 Expression: A New Model for Investigating HIV-1 Transmission and Treatment Efficacy. PLoS ONE. 2013;8(5):e63537. Epub 2013/05/22. doi: 10.1371/journal.pone.0063537. PubMed PMID: 23691059; PubMed Central PMCID: PMC3655194.

6. Stewart A, Harrison JS, Regula LK, Lai JR. Side chain requirements for affinity and specificity in D5, an HIV-1 antibody derived from the VH1-69 germline segment. BMC biochemistry. 2013;14:9. Epub 2013/04/10. doi: 10.1186/1471-2091-14-9. PubMed PMID: 23566198; PubMed Central PMCID: PMC3626704.

7. Williams DW, Calderon TM, Lopez L, Carvallo-Torres L, Gaskill PJ, Eugenin EA, Morgello S, Berman JW. Mechanisms of HIV entry into the CNS: increased sensitivity of HIV infected CD14+CD16+ monocytes to CCL2 and key roles of CCR2, JAM-A, and ALCAM in diapedesis. PLoS ONE. 2013;8(7):e69270. Epub 2013/08/08. doi: 10.1371/journal.pone.0069270. PubMed PMID: 23922698; PubMed Central PMCID: PMC3724935.

8. Zhao R, Chinai JM, Buhl S, Scandiuzzi L, Ray A, Jeon H, Ohaegbulam KC, Ghosh K, Zhao A, Scharff MD, Zang X. HHLA2 is a member of the B7 family and inhibits human CD4 and CD8 T-cell function. Proc Natl Acad Sci U S A. 2013;110(24):9879-84. Epub 2013/05/30. doi: 10.1073/pnas.1303524110. PubMed PMID: 23716685; PubMed Central PMCID: PMC3683785.

 

Core Leadership 

Steven Porcelli, MD
Director
718-430-3228
steven.porcelli@einstein.yu.edu 

Jinghang Zhang, MD
Operations Director
718-430-8787
jinghang.zhang@einstein.yu.edu  

 
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