To learn more about integrating biophotonic techniques into their science, Einstein graduate students may enroll in a course that GLBC members organize and teach.
1032 Quantitative Imaging of Cells
COURSE LEADERS: Drs. Ben Ovryn and Erik Snapp
CREDITS/CLASS MEETINGS: 3 semester hours; Lectures: 1.5 hrs, twice a week plus laboratories: 1.5 hrs, once per week.
ENROLLMENT: Enrollment for the course is limited to approximately 15 students due to restricted lab space. Preference will be given to 2nd year and above graduate and MSTP students with a quantitative imaging component to their projects.
COURSE DESCRIPTION: This course presents an in-depth analysis of the principles and applications of light microscopy as applied to imaging cellular structures and molecules. Topics will include: the essentials of light microscopy; fluorophores; live cell imaging; deconvolution; confocal microscopy; photomanipulation; fluorescence correlation spectroscopy, multi-photon intravital imaging; evanescent wave imaging; fluorescence energy transfer and fluorescence lifetime imaging; FACS and evolving microscopies. Formal lectures will be supplemented with laboratory demonstrations that use state-of-the-art microscopes. Grading will be based upon a combination of laboratory exercises, problem sets, midterm, and a final exam.