Analytical Imaging Facility

Equipment & Techniques

The AIF supports both Electron and Light microscopy. The AIF also has a variety of image analysis programs. Please click on the category of interest.

Electron Microscopy 

  • Transmission Electron Microscopes

    Microscope in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it. 

     

      Highlighted Features: 

      • Cellular structure
      • Electron tomography
      • Single particle analysis
      • Macromolecular localization
      • Host-pathogen interaction
      • Tissue pathology
     
    Header TEM  
     
     
  • Scanning Electron Microscope

    Microscope that produces images of a sample by scanning it with a focused beam of electrons. The electrons interact with atoms in the sample, producing various signals that contain information about the sample's surface topography and composition.

     

      Highlighted Features: 

      • Surface imaging
      • Macromolecular localization
      • Host-pathogen interactions
      • Elemental analysis
      • 3-D reconstruction
      • Correlative Fluorescence and Electron Microscopy (CLEM)
     
    Header SEM  
      
     
  • Specimen Preparation for Electron Microscopy

    The expert staff can design a specimen preparation protocol to optimize cellular preservation tailored to your individual experiment objective. 

       

     
                                 
     
     
 
 

Light Microscopy 

 
 
 

Image Analysis

  • Image Visualization and Quantification

    The AIF provides two high-end computer workstations and supports several image analysis packages that can be used for visualization, quantification and restoration of light microscope and electron microscopy image data. The AIF advises and trains users on proper ways to generate data and perform quantitative data analysis on their own. Image analysis can also be conducted on an assisted basis by the AIF staff.

      Highlighted Features: 

      • Quantify fluorescent intensities, shapes, and volumes
      • Deconvolve epi-fluorescent data sets
      • Track dynamics of moving object
      • Study interactions of multiple populations
      • Convert 3D time-lapse data into movies
      • 3D reconstructions
      • Electron tomography reconstructions
      • Single particle analysis
     
    Equipment analysis volocity  
     
                                                                              
 
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